Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses
Plants display a great diversity of particular cell types that obviously perform functions and regulations that are essential for successful growth and development, whether under optimal or adverse conditions. The functions performed by each of these particular cell types must be associated with spe...
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| Format: | Capítulo de libro |
| Language: | Inglés |
| Published: |
Springer Nature
2023
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| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.11939/8568 https://link.springer.com/protocol/10.1007/978-1-0716-3044-0_19 |
| _version_ | 1855492515553083392 |
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| author | Tadeo, Francisco R. Agustí, Manuel Merelo, Paz Talón, Manuel |
| author2 | Couée, Ivan |
| author_browse | Agustí, Manuel Couée, Ivan Merelo, Paz Tadeo, Francisco R. Talón, Manuel |
| author_facet | Couée, Ivan Tadeo, Francisco R. Agustí, Manuel Merelo, Paz Talón, Manuel |
| author_sort | Tadeo, Francisco R. |
| collection | ReDivia |
| description | Plants display a great diversity of particular cell types that obviously perform functions and regulations that are essential for successful growth and development, whether under optimal or adverse conditions. The functions performed by each of these particular cell types must be associated with specific transcriptomic, proteomic, and metabolic profiles that cannot be disentangled by analyzing whole plant organs and tissues. Laser microdissection is a technique for the collection of specific cell types in plant organs and tissues comprising heterogeneous cell populations. It has been successfully used for physiological and molecular studies. Laser microdissection can be applied to any plant species as long as it is possible to reliably identify the cell types of interest. Here, we describe step by step, using citrus as a model plant, a fast, simple, easy to perform, and experimentally validated protocol to collect cells from the abscission zone, a specific tissue that is difficult to access and whose activity is important in the response of plants to adverse environmental conditions. |
| format | Capítulo de libro |
| id | ReDivia8568 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Springer Nature |
| publisherStr | Springer Nature |
| record_format | dspace |
| spelling | ReDivia85682025-04-25T14:50:13Z Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses Tadeo, Francisco R. Agustí, Manuel Merelo, Paz Talón, Manuel Couée, Ivan Calyx abscission zone Cell separation Cryosectioning Laminar abscission zone Laser microdissection U30 Research methods F30 Plant genetics and breeding A50 Agricultural research Q01 Food science and technology Abscission Citrus Ethylene New technology Plants display a great diversity of particular cell types that obviously perform functions and regulations that are essential for successful growth and development, whether under optimal or adverse conditions. The functions performed by each of these particular cell types must be associated with specific transcriptomic, proteomic, and metabolic profiles that cannot be disentangled by analyzing whole plant organs and tissues. Laser microdissection is a technique for the collection of specific cell types in plant organs and tissues comprising heterogeneous cell populations. It has been successfully used for physiological and molecular studies. Laser microdissection can be applied to any plant species as long as it is possible to reliably identify the cell types of interest. Here, we describe step by step, using citrus as a model plant, a fast, simple, easy to perform, and experimentally validated protocol to collect cells from the abscission zone, a specific tissue that is difficult to access and whose activity is important in the response of plants to adverse environmental conditions. 2023-03-24T09:14:25Z 2023-03-24T09:14:25Z 2023 bookPart Tadeo, F. R., Agustí, J., Merelo, P. & Talón, M. (2023). Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses. In: Couée, I. (ed.) Plant Abiotic Stress Signaling, 365-373. 978-1-0716-3044-0 (eBook ISBN) 978-1-0716-3043-3 (Hardcover ISBN) 978-1-0716-3046-4 (Softcover ISBN) 1064-3745 (Series ISSN) 1940-6029 (Series E-ISSN) https://hdl.handle.net/20.500.11939/8568 10.1007/978-1-0716-3044-0_19 https://link.springer.com/protocol/10.1007/978-1-0716-3044-0_19 en Plant Abiotic Stress Signaling The development of the LMD protocol was supported by grants from the Spanish Instituto Nacional de Investigaciones Agrarias # RTA08-00065-00-00 and # RTA2014-00071-C06-01, the Spanish Ministerio de Educación y Ciencia-FEDER # AGL2007-65437-C04-01/AGR and the Spanish Ministerio de Industria # AGL2011-30240. The preparation of this manuscript was supported by grants from the Spanish Ministerio de Ciencia, Innovación y Universidades # RTI2018-097790-R-100 and from the Instituto Valenciano de Investigaciones Agrarias (Spain), # 51915 and # 52002. info:eu-repo/grantAgreement/MICINN//RTA2008-00065-00-00/ES/Identificación y caracterización molecular de genes asociados a la producción y la calidad del fruto de los cítricos info:eu-repo/grantAgreement/MINECO//RTA2014-00071-C06-01/ES/Fenotipado, características Agronómicas y estructura genómica de las nuevas variedades de cítricos/ info:eu-repo/grantAgreement/MEC//AGL2007-65437-C04-01/ES/ANALISIS GENOMICO Y FENOTIPICO DE LA VARIABILIDAD GENETICA DE LOS CITRICOS EN RELACION CON LA TOLERANCIA FRENTE A LOS ESTRESES ABIOTICOS/ info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51915 info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE info:eu-repo/grantAgreement/MICINN//AGL2011-30240/ES/IDENTIFICACION Y CARACTERIZACION DE GENES RELACIONADOS CON LA CALIDAD DE LA FRUTA Y LA PRODUCTIVIDAD EN CITRICOS MEDIANTE TECNICAS DE GENOMICA Y METABOLOMICA/ info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-097790-R-I00/ES/DESARROLLO Y OBTENCION DE NUEVAS VARIEDADES DE CITRICOS MEDIANTE LA APLICACION DE HERRAMIENTAS GENOMICAS DE MEJORA BASADAS EN LOS METODOS DE SECUENCIACION DE NUEVA GENERACION Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer Nature electronico |
| spellingShingle | Calyx abscission zone Cell separation Cryosectioning Laminar abscission zone Laser microdissection U30 Research methods F30 Plant genetics and breeding A50 Agricultural research Q01 Food science and technology Abscission Citrus Ethylene New technology Tadeo, Francisco R. Agustí, Manuel Merelo, Paz Talón, Manuel Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title | Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title_full | Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title_fullStr | Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title_full_unstemmed | Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title_short | Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses |
| title_sort | laser microdissection a high precision approach to isolate specific cell types from any plant species for downstream molecular analyses |
| topic | Calyx abscission zone Cell separation Cryosectioning Laminar abscission zone Laser microdissection U30 Research methods F30 Plant genetics and breeding A50 Agricultural research Q01 Food science and technology Abscission Citrus Ethylene New technology |
| url | https://hdl.handle.net/20.500.11939/8568 https://link.springer.com/protocol/10.1007/978-1-0716-3044-0_19 |
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