IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers
Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thalia...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
BMC
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7386 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02994-8 |
| Sumario: | Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the
detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at
specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and
cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION
(IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC
EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling
module has not yet been demonstrated in other plant species.
Results: The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base
of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana
benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on
plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an
observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the
preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in
normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A
at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of
both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and
Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic
processing machinery was unable to yield active peptides. Conclusion: Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like
family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an
adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our
results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in
angiosperms. |
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