Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties

Chemical characterization of clementine varieties (Citrus clementina Hort. ex Tan.) essential oils (EO) can lead to variety identification and valorization of their potential use in food and aroma industries. The goal of this study was the chemometric discrimination between two very closely related...

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Autores principales: González-Mas, María C., Rambla, Jose L., Gómez-Cadenas, Aurelio, Blázquez, María Amparo, López-Gresa, María P., Granell, Antonio
Formato: Artículo
Lenguaje:Inglés
Publicado: MDPI 2022
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/7873
https://www.mdpi.com/2223-7747/10/9/1789
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author González-Mas, María C.
Rambla, Jose L.
Gómez-Cadenas, Aurelio
Blázquez, María Amparo
López-Gresa, María P.
Granell, Antonio
author_browse Blázquez, María Amparo
González-Mas, María C.
Granell, Antonio
Gómez-Cadenas, Aurelio
López-Gresa, María P.
Rambla, Jose L.
author_facet González-Mas, María C.
Rambla, Jose L.
Gómez-Cadenas, Aurelio
Blázquez, María Amparo
López-Gresa, María P.
Granell, Antonio
author_sort González-Mas, María C.
collection ReDivia
description Chemical characterization of clementine varieties (Citrus clementina Hort. ex Tan.) essential oils (EO) can lead to variety identification and valorization of their potential use in food and aroma industries. The goal of this study was the chemometric discrimination between two very closely related and morphologically identical clementine varieties, Clemenules (NL) and Clemenpons (PO), based on their rind EO, to identify the differential volatile organic compounds (VOCs) and to determine their antioxidant capacity. EO rind volatile profile was determined by gas chromatography coupled to mass spectrometry in Citrus fruit at different ripening stages grown two independent years in two different locations. Untargeted metabolomics and multivariate data analysis showed an evolution of EO volatile profiles markedly parallel in both varieties. Although EO qualitative composition was identical in both varieties, PLS-DA allowed the identification of characteristic VOCs, quantitatively discriminating them along all the ripening process. PO showed higher accumulation of several mono- and sesquiterpene compounds such as trans-carveol, while NL showed higher levels of aldehyde and alcohol non-terpenoids like dodecanal. Both varieties evinced identical EO antioxidant activities, indicating a similar value for food preservation. Hence, untargeted metabolomics approach based on rind EO volatiles was revealed as a powerful technique able to differentiate between morphologically undistinguishable Citrus varieties.
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spelling ReDivia78732025-04-25T14:48:38Z Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties González-Mas, María C. Rambla, Jose L. Gómez-Cadenas, Aurelio Blázquez, María Amparo López-Gresa, María P. Granell, Antonio Citrus clementina Clemenules Clemenpons Flavedo Volatile organics compounds Antioxidant activity F60 Plant physiology and biochemistry U30 Research methods F30 Plant genetics and breeding Q01 Food science and technology Chemical characterization of clementine varieties (Citrus clementina Hort. ex Tan.) essential oils (EO) can lead to variety identification and valorization of their potential use in food and aroma industries. The goal of this study was the chemometric discrimination between two very closely related and morphologically identical clementine varieties, Clemenules (NL) and Clemenpons (PO), based on their rind EO, to identify the differential volatile organic compounds (VOCs) and to determine their antioxidant capacity. EO rind volatile profile was determined by gas chromatography coupled to mass spectrometry in Citrus fruit at different ripening stages grown two independent years in two different locations. Untargeted metabolomics and multivariate data analysis showed an evolution of EO volatile profiles markedly parallel in both varieties. Although EO qualitative composition was identical in both varieties, PLS-DA allowed the identification of characteristic VOCs, quantitatively discriminating them along all the ripening process. PO showed higher accumulation of several mono- and sesquiterpene compounds such as trans-carveol, while NL showed higher levels of aldehyde and alcohol non-terpenoids like dodecanal. Both varieties evinced identical EO antioxidant activities, indicating a similar value for food preservation. Hence, untargeted metabolomics approach based on rind EO volatiles was revealed as a powerful technique able to differentiate between morphologically undistinguishable Citrus varieties. 2022-01-21T18:02:21Z 2022-01-21T18:02:21Z 2021 article publishedVersion González-Mas, M. C., Rambla, J. L., Gómez-Cadenas, A., Blázquez, M. A., López-Gresa, M. P. & Granell, A. (2021). Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties. Plants, 10(9), 1789. 2223-7747 http://hdl.handle.net/20.500.11939/7873 10.3390/plants10091789 https://www.mdpi.com/2223-7747/10/9/1789 en info:eu-repo/grantAgreement/EC/H2020/679796 info:eu-repo/grantAgreement/EC/H2020/634561 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BIO2016-78601-R This work was funded by the European Commission H2020 research and innovation program through the TOMGEM project (679796.), TRADITOM project (634561), SusCrop-ERA-NET proposal SOLNUE, and Spanish Ministry project BIO2016-78601-R. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess MDPI electronico
spellingShingle Citrus clementina
Clemenules
Clemenpons
Flavedo
Volatile organics compounds
Antioxidant activity
F60 Plant physiology and biochemistry
U30 Research methods
F30 Plant genetics and breeding
Q01 Food science and technology
González-Mas, María C.
Rambla, Jose L.
Gómez-Cadenas, Aurelio
Blázquez, María Amparo
López-Gresa, María P.
Granell, Antonio
Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title_full Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title_fullStr Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title_full_unstemmed Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title_short Untargeted Metabolomics of Rind Essential Oils Allowed to Differentiate Two Closely Related Clementine Varieties
title_sort untargeted metabolomics of rind essential oils allowed to differentiate two closely related clementine varieties
topic Citrus clementina
Clemenules
Clemenpons
Flavedo
Volatile organics compounds
Antioxidant activity
F60 Plant physiology and biochemistry
U30 Research methods
F30 Plant genetics and breeding
Q01 Food science and technology
url http://hdl.handle.net/20.500.11939/7873
https://www.mdpi.com/2223-7747/10/9/1789
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