The Citrus Quarantine Station in Spain

A Citrus Quarantine Station has been recently established in Spain. It is based in a tissue culture procedure developed for safe budwood introduction, consisting of the following steps: a) preliminary budwood inspection; b) budwood disinfestation; c) budwood culture in vitro at 32 C for flushing; d)...

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Autores principales: Navarro, Luis, Juárez, José, Pina, José A., Ballester-Olmos, José F.
Otros Autores: Garnsey, Stephen M.
Formato: conferenceObject
Lenguaje:Inglés
Publicado: iocv 2022
Materias:
Acceso en línea:http://hdl.handle.net/20.500.11939/7867
https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th365_370.pdf
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author Navarro, Luis
Juárez, José
Pina, José A.
Ballester-Olmos, José F.
author2 Garnsey, Stephen M.
author_browse Ballester-Olmos, José F.
Garnsey, Stephen M.
Juárez, José
Navarro, Luis
Pina, José A.
author_facet Garnsey, Stephen M.
Navarro, Luis
Juárez, José
Pina, José A.
Ballester-Olmos, José F.
author_sort Navarro, Luis
collection ReDivia
description A Citrus Quarantine Station has been recently established in Spain. It is based in a tissue culture procedure developed for safe budwood introduction, consisting of the following steps: a) preliminary budwood inspection; b) budwood disinfestation; c) budwood culture in vitro at 32 C for flushing; d) disinfestation of flushes obtained; e) isolation of 0.1-0.2 mm long shoot tips; f) grafting shoot tips in vitro; g) transplanting micrografted plants to a quarantine greenhouse; h) indexing of micrografted plants; i) release of budwood. Any abnormal or contaminated tissue or plant found during the process and all introduced plant material except the shoot tips are destroyed. Pests, fungi, bacteria and even virus and virus-like diseases introduced with the original material can be easily eliminated by this procedure. The estimated time from budwood introduction until budwood release is 24-30 months, including 18-24 months for xyloporosis indexing.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia78672025-04-25T14:52:49Z The Citrus Quarantine Station in Spain Navarro, Luis Juárez, José Pina, José A. Ballester-Olmos, José F. Garnsey, Stephen M. Shoot-tip grafting In vitro citrus diseases H20 Plant diseases U30 Research methods Q02 Food processing and preservation J10 Handling, transport, storage and protection of agricultural products Tissue culture Virus free plants A Citrus Quarantine Station has been recently established in Spain. It is based in a tissue culture procedure developed for safe budwood introduction, consisting of the following steps: a) preliminary budwood inspection; b) budwood disinfestation; c) budwood culture in vitro at 32 C for flushing; d) disinfestation of flushes obtained; e) isolation of 0.1-0.2 mm long shoot tips; f) grafting shoot tips in vitro; g) transplanting micrografted plants to a quarantine greenhouse; h) indexing of micrografted plants; i) release of budwood. Any abnormal or contaminated tissue or plant found during the process and all introduced plant material except the shoot tips are destroyed. Pests, fungi, bacteria and even virus and virus-like diseases introduced with the original material can be easily eliminated by this procedure. The estimated time from budwood introduction until budwood release is 24-30 months, including 18-24 months for xyloporosis indexing. 2022-01-21T17:59:54Z 2022-01-21T17:59:54Z 1984 conferenceObject Navarro, L., Juarez, J., Pina, J. A. & Ballester-Olmos, J. F. (1984). The Citrus Quarantine Station in Spain. Proceedings of the Ninth Conference of the IOCV, 365-370 2313-5123 http://hdl.handle.net/20.500.11939/7867 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th365_370.pdf en 1983-05 Ninth Conference of the International Organization of Citrus Virologists Argentina Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess iocv electronico
spellingShingle Shoot-tip grafting
In vitro
citrus diseases
H20 Plant diseases
U30 Research methods
Q02 Food processing and preservation
J10 Handling, transport, storage and protection of agricultural products
Tissue culture
Virus free plants
Navarro, Luis
Juárez, José
Pina, José A.
Ballester-Olmos, José F.
The Citrus Quarantine Station in Spain
title The Citrus Quarantine Station in Spain
title_full The Citrus Quarantine Station in Spain
title_fullStr The Citrus Quarantine Station in Spain
title_full_unstemmed The Citrus Quarantine Station in Spain
title_short The Citrus Quarantine Station in Spain
title_sort citrus quarantine station in spain
topic Shoot-tip grafting
In vitro
citrus diseases
H20 Plant diseases
U30 Research methods
Q02 Food processing and preservation
J10 Handling, transport, storage and protection of agricultural products
Tissue culture
Virus free plants
url http://hdl.handle.net/20.500.11939/7867
https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/9th365_370.pdf
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