Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike
Currently, kiwifruit cultivation arouses great economic interest in the agricultural sector in several countries of the European Union due to high consumer demand and good results achieved in terms of production potential and fruit quality. One of the main bacterial species that cause yield losses i...
| Autores principales: | , , , , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
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MDPI
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7811 https://www.mdpi.com/2073-4395/11/12/2464 |
| _version_ | 1855032676591861760 |
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| author | Morán, Félix Marco-Noales, Ester Landeras, Elena Roselló, Montserrat Abelleira, Adela Gonzalez, Ana López, María M. |
| author_browse | Abelleira, Adela Gonzalez, Ana Landeras, Elena López, María M. Marco-Noales, Ester Morán, Félix Roselló, Montserrat |
| author_facet | Morán, Félix Marco-Noales, Ester Landeras, Elena Roselló, Montserrat Abelleira, Adela Gonzalez, Ana López, María M. |
| author_sort | Morán, Félix |
| collection | ReDivia |
| description | Currently, kiwifruit cultivation arouses great economic interest in the agricultural sector in several countries of the European Union due to high consumer demand and good results achieved in terms of production potential and fruit quality. One of the main bacterial species that cause yield losses in kiwifruit plants is Pseudomonas syringae. Diseases such as bacterial canker, caused by pathovar (pv.) actinidiae; floral bud necrosis caused by pv. syringae and leaf spots caused by pv. actinidifoliorum (Pfm) are clear examples. Between 2014 and 2017, in the main kiwifruit producing areas in the north and east of Spain, several surveys were carried out in search of these pathogens. Analyses realized from symptomatic and asymptomatic plants of Actinidia deliciosa revealed the existence of new bacterial isolates close to Pfm. These new isolates werelow virulence pathogens similar to Pfm but belonging to a new group of P. syringae that affected the leaves of A. chinensis var. deliciosa. This study focused on the characterization and classification of these new isolates by a polyphasic approach in order to provide more information for understanding how the different populations of P. syringae affecting kiwifruit. They had the phenotypic characteristics of Pfm but by molecular approaches, they constituted a supported genetic lineage closely-related to Pfm independent of the five lineages described so far. This work revealed the great diversity found in P. syringae species
affecting kiwifruit plants and supports the hypothesis that Pfm is a low virulence pathogen which is long established in Europe. |
| format | publishedVersion |
| id | ReDivia7811 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia78112025-04-25T14:48:35Z Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike Morán, Félix Marco-Noales, Ester Landeras, Elena Roselló, Montserrat Abelleira, Adela Gonzalez, Ana López, María M. pathovars MLSA virulence classification linage H20 Plant diseases Actinidia deliciosa Pseudomonas syringae Currently, kiwifruit cultivation arouses great economic interest in the agricultural sector in several countries of the European Union due to high consumer demand and good results achieved in terms of production potential and fruit quality. One of the main bacterial species that cause yield losses in kiwifruit plants is Pseudomonas syringae. Diseases such as bacterial canker, caused by pathovar (pv.) actinidiae; floral bud necrosis caused by pv. syringae and leaf spots caused by pv. actinidifoliorum (Pfm) are clear examples. Between 2014 and 2017, in the main kiwifruit producing areas in the north and east of Spain, several surveys were carried out in search of these pathogens. Analyses realized from symptomatic and asymptomatic plants of Actinidia deliciosa revealed the existence of new bacterial isolates close to Pfm. These new isolates werelow virulence pathogens similar to Pfm but belonging to a new group of P. syringae that affected the leaves of A. chinensis var. deliciosa. This study focused on the characterization and classification of these new isolates by a polyphasic approach in order to provide more information for understanding how the different populations of P. syringae affecting kiwifruit. They had the phenotypic characteristics of Pfm but by molecular approaches, they constituted a supported genetic lineage closely-related to Pfm independent of the five lineages described so far. This work revealed the great diversity found in P. syringae species affecting kiwifruit plants and supports the hypothesis that Pfm is a low virulence pathogen which is long established in Europe. 2021-12-09T15:50:03Z 2021-12-09T15:50:03Z 2021 publishedVersion Morán, F., Marco-Noales, E., Landeras, E., Roselló, M., Abelleira, A., Gonzalez, A. J. et al. (2021). Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike. Agronomy, 11(12): 2464. https://doi.org/10.3390/agronomy11122464 2073-4395 http://hdl.handle.net/20.500.11939/7811 10.3390/agronomy11122464 https://www.mdpi.com/2073-4395/11/12/2464 en Info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTA2013-00072-C03-03 E-RTA2013-00072-C03-03 EL CHANCRO BACTERIANO DE KIWI, CAUSADO POR LA BACTERIA EMERGENTE PSEUDOMONAS SYRINGAE PV. ACTINIDAE: SITUACIÓN EN LA COMUNIDAD VALENCIANA, MÉTODOS DE DIAGNOSTICO Y CARACTERIZACIÓN DE CEPAS openAccess MDPI electronico |
| spellingShingle | pathovars MLSA virulence classification linage H20 Plant diseases Actinidia deliciosa Pseudomonas syringae Morán, Félix Marco-Noales, Ester Landeras, Elena Roselló, Montserrat Abelleira, Adela Gonzalez, Ana López, María M. Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title | Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title_full | Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title_fullStr | Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title_full_unstemmed | Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title_short | Polyphasic Analysis of Isolates from Kiwifruit Reveal New Genetic Lineages of Pseudomonas syringae pv. actinidifoliorum Look-Alike |
| title_sort | polyphasic analysis of isolates from kiwifruit reveal new genetic lineages of pseudomonas syringae pv actinidifoliorum look alike |
| topic | pathovars MLSA virulence classification linage H20 Plant diseases Actinidia deliciosa Pseudomonas syringae |
| url | http://hdl.handle.net/20.500.11939/7811 https://www.mdpi.com/2073-4395/11/12/2464 |
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