IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers
Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thalia...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
BMC
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7386 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02994-8 |
| _version_ | 1855492275224707072 |
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| author | Ventimilla, Daniel Velázquez, Karelia Ruiz-Ruiz, Susana Terol, Javier Pérez-Amador, Miguel A. Vives, María C. Guerri, José Talón, Manuel Tadeo, Francisco R. |
| author_browse | Guerri, José Pérez-Amador, Miguel A. Ruiz-Ruiz, Susana Tadeo, Francisco R. Talón, Manuel Terol, Javier Velázquez, Karelia Ventimilla, Daniel Vives, María C. |
| author_facet | Ventimilla, Daniel Velázquez, Karelia Ruiz-Ruiz, Susana Terol, Javier Pérez-Amador, Miguel A. Vives, María C. Guerri, José Talón, Manuel Tadeo, Francisco R. |
| author_sort | Ventimilla, Daniel |
| collection | ReDivia |
| description | Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the
detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at
specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and
cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION
(IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC
EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling
module has not yet been demonstrated in other plant species.
Results: The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base
of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana
benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on
plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an
observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the
preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in
normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A
at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of
both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and
Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic
processing machinery was unable to yield active peptides. Conclusion: Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like
family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an
adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our
results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in
angiosperms. |
| format | Artículo |
| id | ReDivia7386 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | BMC |
| publisherStr | BMC |
| record_format | dspace |
| spelling | ReDivia73862025-04-25T14:48:17Z IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers Ventimilla, Daniel Velázquez, Karelia Ruiz-Ruiz, Susana Terol, Javier Pérez-Amador, Miguel A. Vives, María C. Guerri, José Talón, Manuel Tadeo, Francisco R. Abscission Zone Abscission signaling module Cell separation Gene silencing and overexpression Hormone peptide LRR-RLKs F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction H20 Plant diseases Solanaceae Background: Abscission is an active, organized, and highly coordinated cell separation process enabling the detachment of aerial organs through the modification of cell-to-cell adhesion and breakdown of cell walls at specific sites on the plant body known as abscission zones. In Arabidopsis thaliana, abscission of floral organs and cauline leaves is regulated by the interaction of the hormonal peptide INFLORESCENCE DEFICIENT IN ABSCISSION (IDA), a pair of redundant receptor-like protein kinases, HAESA (HAE) and HAESA-LIKE2 (HSL2), and SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) co-receptors. However, the functionality of this abscission signaling module has not yet been demonstrated in other plant species. Results: The expression of the pair of NbenIDA1 homeologs and the receptor NbenHAE.1 was supressed at the base of the corolla tube by the inoculation of two virus-induced gene silencing (VIGS) constructs in Nicotiana benthamiana. These gene suppression events arrested corolla abscission but did not produce any obvious effect on plant growth. VIGS plants retained a higher number of corollas attached to the flowers than control plants, an observation related to a greater corolla breakstrength. The arrest of corolla abscission was associated with the preservation of the parenchyma tissue at the base of the corolla tube that, in contrast, was virtually collapsed in normal corollas. In contrast, the inoculation of a viral vector construct that increased the expression of NbenIDA1A at the base of the corolla tube negatively affected the growth of the inoculated plants accelerating the timing of both corolla senescence and abscission. However, the heterologous ectopic overexpression of citrus CitIDA3 and Arabidopsis AtIDA in N. benthamiana did not alter the standard plant phenotype suggesting that the proteolytic processing machinery was unable to yield active peptides. Conclusion: Here, we demonstrate that the pair of NbenIDA1 homeologs encoding small peptides of the IDA-like family and the receptor NbenHAE.1 control cellular breakdown at the base of the corolla tube awhere an adventitious AZ should be formed and, therefore, corolla abscission in N. benthamiana flowers. Altogether, our results provide the first evidence supporting the notion that the IDA-HAE/HSL2 signaling module is conserved in angiosperms. 2021-05-26T10:26:15Z 2021-05-26T10:26:15Z 2021 article publishedVersion Ventimilla, D., Velázquez, K., Ruiz-Ruiz, S., Terol, J., Pérez-Amador, M. A., Vives, M. C. et al (2021). IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)- like receptors regulate corolla abscission in Nicotiana benthamiana flowers. BMC Plant Biology, 21, 226. 1471-2229 http://hdl.handle.net/20.500.11939/7386 10.1186/s12870-021-02994-8 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02994-8 en Info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/RTI2018-097790-R-100 Info:eu-repo/grantAgreement/ERDF/POCV 2014-2020/51915 Financial support was provided by the Ministerio de Ciencia, Innovación y Universidades (Spain) trough grant # RTI2018-097790-R-100 and from the Instituto Valenciano de Investigaciones Agrarias (Spain), through grants 51915 and 52002. Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ openAccess BMC electronico |
| spellingShingle | Abscission Zone Abscission signaling module Cell separation Gene silencing and overexpression Hormone peptide LRR-RLKs F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction H20 Plant diseases Solanaceae Ventimilla, Daniel Velázquez, Karelia Ruiz-Ruiz, Susana Terol, Javier Pérez-Amador, Miguel A. Vives, María C. Guerri, José Talón, Manuel Tadeo, Francisco R. IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title_full | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title_fullStr | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title_full_unstemmed | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title_short | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like peptides and HAE (HAESA)-like receptors regulate corolla abscission in Nicotiana benthamiana flowers |
| title_sort | ida inflorescence deficient in abscission like peptides and hae haesa like receptors regulate corolla abscission in nicotiana benthamiana flowers |
| topic | Abscission Zone Abscission signaling module Cell separation Gene silencing and overexpression Hormone peptide LRR-RLKs F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction H20 Plant diseases Solanaceae |
| url | http://hdl.handle.net/20.500.11939/7386 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-021-02994-8 |
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