Ethylene-induced differential gene expression during abscission of citrus leaves
The main objective of this work was to identify and classify genes involved in the process of leaf abscission in Clementina de Nules (Citrus clementina Hort. Ex Tan.). A 7 K unigene citrus cDNA microarray containing 12 K spots was used to characterize the transcriptome of the ethylene-induced a...
| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
Oxford University Press
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/6432 https://academic.oup.com/jxb/article/59/10/2717/439660 |
| _version_ | 1855032425859514368 |
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| author | Agustí, Javier Merelo, Paz Cercós, Manuel Tadeo, Francisco R. Talón, Manuel |
| author_browse | Agustí, Javier Cercós, Manuel Merelo, Paz Tadeo, Francisco R. Talón, Manuel |
| author_facet | Agustí, Javier Merelo, Paz Cercós, Manuel Tadeo, Francisco R. Talón, Manuel |
| author_sort | Agustí, Javier |
| collection | ReDivia |
| description | The main objective of this work was to identify and
classify genes involved in the process of leaf abscission
in Clementina de Nules (Citrus clementina Hort.
Ex Tan.). A 7 K unigene citrus cDNA microarray
containing 12 K spots was used to characterize the
transcriptome of the ethylene-induced abscission process
in laminar abscission zone-enriched tissues and
the petiole of debladed leaf explants. In these conditions,
ethylene induced 100% leaf explant abscission
in 72 h while, in air-treated samples, the abscission
period started later and took 240 h. Gene expression
monitored during the first 36 h of ethylene treatment
showed that out of the 12 672 cDNA microarray
probes, ethylene differentially induced 725 probes
distributed as follows: 216 (29.8%) probes in the
laminar abscission zone and 509 (70.2%) in the petiole.
Functional MIPS classification and manual annotation
of differentially expressed genes highlighted key processes
regulating the activation and progress of the
cell separation that brings about abscission. These
included cell-wall modification, lipid transport, protein
biosynthesis and degradation, and differential activation
of signal transduction and transcription control
pathways. Expression data associated with the petiole
indicated the occurrence of a double defensive strategy
mediated by the activation of a biochemical programme
including scavenging ROS, defence and PR
genes, and a physical response mostly based on lignin
biosynthesis and deposition. This work identifies new
genes probably involved in the onset and development
of the leaf abscission process and suggests a different
but co-ordinated and complementary role for the
laminar abscission zone and the petiole during the
process of abscission. |
| format | article |
| id | ReDivia6432 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | ReDivia64322025-04-25T14:47:04Z Ethylene-induced differential gene expression during abscission of citrus leaves Agustí, Javier Merelo, Paz Cercós, Manuel Tadeo, Francisco R. Talón, Manuel cDNA microarray Expression profiling Laminar abscission zone F30 Plant genetics and breeding Clementines Mandarins Petioles Citrus The main objective of this work was to identify and classify genes involved in the process of leaf abscission in Clementina de Nules (Citrus clementina Hort. Ex Tan.). A 7 K unigene citrus cDNA microarray containing 12 K spots was used to characterize the transcriptome of the ethylene-induced abscission process in laminar abscission zone-enriched tissues and the petiole of debladed leaf explants. In these conditions, ethylene induced 100% leaf explant abscission in 72 h while, in air-treated samples, the abscission period started later and took 240 h. Gene expression monitored during the first 36 h of ethylene treatment showed that out of the 12 672 cDNA microarray probes, ethylene differentially induced 725 probes distributed as follows: 216 (29.8%) probes in the laminar abscission zone and 509 (70.2%) in the petiole. Functional MIPS classification and manual annotation of differentially expressed genes highlighted key processes regulating the activation and progress of the cell separation that brings about abscission. These included cell-wall modification, lipid transport, protein biosynthesis and degradation, and differential activation of signal transduction and transcription control pathways. Expression data associated with the petiole indicated the occurrence of a double defensive strategy mediated by the activation of a biochemical programme including scavenging ROS, defence and PR genes, and a physical response mostly based on lignin biosynthesis and deposition. This work identifies new genes probably involved in the onset and development of the leaf abscission process and suggests a different but co-ordinated and complementary role for the laminar abscission zone and the petiole during the process of abscission. 2020-05-08T16:26:24Z 2020-05-08T16:26:24Z 2008 article publishedVersion Agustí, J., Merelo, P., Cercós, M., Tadeo, F. R., & Talón, M. (2008). Ethylene-induced differential gene expression during abscission of citrus leaves. Journal of experimental botany, 59(10), 2717-2733. 0022-0957 http://hdl.handle.net/20.500.11939/6432 10.1093/jxb/ern138 https://academic.oup.com/jxb/article/59/10/2717/439660 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Oxford University Press electronico |
| spellingShingle | cDNA microarray Expression profiling Laminar abscission zone F30 Plant genetics and breeding Clementines Mandarins Petioles Citrus Agustí, Javier Merelo, Paz Cercós, Manuel Tadeo, Francisco R. Talón, Manuel Ethylene-induced differential gene expression during abscission of citrus leaves |
| title | Ethylene-induced differential gene expression during abscission of citrus leaves |
| title_full | Ethylene-induced differential gene expression during abscission of citrus leaves |
| title_fullStr | Ethylene-induced differential gene expression during abscission of citrus leaves |
| title_full_unstemmed | Ethylene-induced differential gene expression during abscission of citrus leaves |
| title_short | Ethylene-induced differential gene expression during abscission of citrus leaves |
| title_sort | ethylene induced differential gene expression during abscission of citrus leaves |
| topic | cDNA microarray Expression profiling Laminar abscission zone F30 Plant genetics and breeding Clementines Mandarins Petioles Citrus |
| url | http://hdl.handle.net/20.500.11939/6432 https://academic.oup.com/jxb/article/59/10/2717/439660 |
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