Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress
IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-...
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| Format: | Artículo |
| Language: | Inglés |
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Springer
2020
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| Online Access: | http://hdl.handle.net/20.500.11939/6353 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-2250-8 |
| _version_ | 1855492067979952128 |
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| author | Ventimilla, Daniel Domingo, Concha González-Ibeas, Daniel Talón, Manuel Tadeo, Francisco R. |
| author_browse | Domingo, Concha González-Ibeas, Daniel Tadeo, Francisco R. Talón, Manuel Ventimilla, Daniel |
| author_facet | Ventimilla, Daniel Domingo, Concha González-Ibeas, Daniel Talón, Manuel Tadeo, Francisco R. |
| author_sort | Ventimilla, Daniel |
| collection | ReDivia |
| description | IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of the IDA-like and HAE-like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploid Nicotiana benthamiana, in order to identify members involved in abscission, stem growth and in the response to drought conditions.
Results
The phylogenetic relationships among the IDA-like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, although NbenIDA2A lacked the hormonal regulatory elements. Expression analyses showed that the pair of NbenIDA1 homeologs were upregulated during corolla abscission. NbenIDA1 and NbenIDA2 pairs showed tissue differential expression under water stress conditions, since NbenIDA1 homeologs were highly expressed in stressed leaves while NbenIDA2 homeologs, especially NbenIDA2B, were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of the IDA-like family and their putative HAE-like receptors.
Conclusion
Our results suggest that the pair of NbenIDA1 homeologs are involved in the natural process of corolla abscission while both pairs of NbenIDA1 and NbenIDA2 homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae. |
| format | Artículo |
| id | ReDivia6353 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia63532025-04-25T14:46:53Z Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress Ventimilla, Daniel Domingo, Concha González-Ibeas, Daniel Talón, Manuel Tadeo, Francisco R. Cell separation Cis-acting regulatory elements LRR-RLKs Signaling peptide Vegetative growth F30 Plant genetics and breeding Abiotic stress Gene expression Phylogeny Solanaceae IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like signaling peptides and the associated HAE (HAESA)-like family of receptor kinases were originally reported in the model plant Arabidopsis thaliana (Arabidopsis) to be deeply involved in the regulation of abscission. Actually, IDA peptides, as cell-to-cell communication elements, appear to be implicated in many developmental processes that rely on cell separation events, and even in the responses to abiotic stresses. However, the knowledge related to the molecular machinery regulating abscission in economically important crops is scarce. In this work, we determined the conservation and phylogeny of the IDA-like and HAE-like gene families in relevant species of the Solanaceae family and analyzed the expression of these genes in the allopolyploid Nicotiana benthamiana, in order to identify members involved in abscission, stem growth and in the response to drought conditions. Results The phylogenetic relationships among the IDA-like members of the Solanaceae studied, grouped the two pairs of NbenIDA1 and NbenIDA2 protein homeologs with the Arabidopsis prepropeptides related to abscission. Analysis of promoter regions searching for regulatory elements showed that these two pairs of homeologs contained both hormonal and drought response elements, although NbenIDA2A lacked the hormonal regulatory elements. Expression analyses showed that the pair of NbenIDA1 homeologs were upregulated during corolla abscission. NbenIDA1 and NbenIDA2 pairs showed tissue differential expression under water stress conditions, since NbenIDA1 homeologs were highly expressed in stressed leaves while NbenIDA2 homeologs, especially NbenIDA2B, were highly expressed in stressed roots. In non-stressed active growing plants, nodes and internodes were the tissues with the highest expression levels of all members of the IDA-like family and their putative HAE-like receptors. Conclusion Our results suggest that the pair of NbenIDA1 homeologs are involved in the natural process of corolla abscission while both pairs of NbenIDA1 and NbenIDA2 homeologs are implicated in the response to water stress. The data also suggest that IDA peptides may be important during stem growth and development. These results provide additional evidence that the functional module formed by IDA peptides and its receptor kinases, as defined in Arabidopsis, may also be conserved in Solanaceae. 2020-04-01T17:22:44Z 2020-04-01T17:22:44Z 2020 article publishedVersion Ventimilla, D., Domingo, C., González-Ibeas, D., Talon, M., & Tadeo, F. R. (2020). Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)-like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress. BMC Plant Biology, 20(1), 34. 1471-2229 http://hdl.handle.net/20.500.11939/6353 10.1186/s12870-020-2250-8 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-2250-8 en Atribución-NoComercial-SinDerivadas 3.0 España http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Springer electronico |
| spellingShingle | Cell separation Cis-acting regulatory elements LRR-RLKs Signaling peptide Vegetative growth F30 Plant genetics and breeding Abiotic stress Gene expression Phylogeny Solanaceae Ventimilla, Daniel Domingo, Concha González-Ibeas, Daniel Talón, Manuel Tadeo, Francisco R. Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title | Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title_full | Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title_fullStr | Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title_full_unstemmed | Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title_short | Differential expression of IDA (INFLORESCENCE DEFICIENT IN ABSCISSION)- like genes in Nicotiana benthamiana during corolla abscission, stem growth and water stress |
| title_sort | differential expression of ida inflorescence deficient in abscission like genes in nicotiana benthamiana during corolla abscission stem growth and water stress |
| topic | Cell separation Cis-acting regulatory elements LRR-RLKs Signaling peptide Vegetative growth F30 Plant genetics and breeding Abiotic stress Gene expression Phylogeny Solanaceae |
| url | http://hdl.handle.net/20.500.11939/6353 https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-020-2250-8 |
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