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One-step multiplex quantitative RT-PCR for the simultaneous detection of viroids and phytoplasmas of pome fruit trees

A one-step multiplex real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan chemistry was developed for the simultaneous detection of Pear blister canker viroid and Apple scar skin viroid along with universal detection of phytoplasmas, in pome trees. Total n...

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Main Authors: Malandraki, Ioanna, Varveri, Christina, Olmos, Antonio, Vassilakos, Nikon
Format: Artículo
Language:Inglés
Published: 2017
Subjects:
Real-time PCR
MGB probes
Apple scar skin viroid
Pear blister canker viroid
'Candidatus Phytoplasma mali'
'Candidatus Phytoplasma pyri'
POLYMERASE-CHAIN-REACTION
REACTION ASSAYS
IDENTIFICATION
SEQUENCE
VIRUSES
DIFFERENTIATION
VALIDATION
ORGANISMS
PLANTS
Online Access:http://hdl.handle.net/20.500.11939/5556

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