Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection

Single chain variable fragments (scFv) specific to the coat protein (CP) (scFv4C) and the RNA replicase NIb (scFv2A) from Plum pox virus (PPV) were engineered. Diverse expression versions addressed to different cell compartments were produced by addition of several peptides. Two cytosolic versions o...

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Bibliographic Details
Main Authors:	Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio
Other Authors:	Llácer, Gerardo
Format:	conferenceObject
Language:	Inglés
Published:	2017
Online Access:	http://hdl.handle.net/20.500.11939/5282

Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA by Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Published 2017

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Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

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Real-time RT-PCR for quantitative detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

Published 2017

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Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction by Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

Published 2017

Get full text

conferenceObject

Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection

Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA by Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

Real-time RT-PCR for quantitative detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano

Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction by Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

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