Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection
Single chain variable fragments (scFv) specific to the coat protein (CP) (scFv4C) and the RNA replicase NIb (scFv2A) from Plum pox virus (PPV) were engineered. Diverse expression versions addressed to different cell compartments were produced by addition of several peptides. Two cytosolic versions o...
| Autores principales: | , , , |
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| Otros Autores: | |
| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5282 |
| _version_ | 1855032253725278208 |
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| author | Gil, Maite Esteban, Olga Martínez, M. Carmen Garcia, Juan Antonio |
| author2 | Llácer, Gerardo |
| author_browse | Esteban, Olga Garcia, Juan Antonio Gil, Maite Llácer, Gerardo Martínez, M. Carmen |
| author_facet | Llácer, Gerardo Gil, Maite Esteban, Olga Martínez, M. Carmen Garcia, Juan Antonio |
| author_sort | Gil, Maite |
| collection | ReDivia |
| description | Single chain variable fragments (scFv) specific to the coat protein (CP) (scFv4C) and the RNA replicase NIb (scFv2A) from Plum pox virus (PPV) were engineered. Diverse expression versions addressed to different cell compartments were produced by addition of several peptides. Two cytosolic versions of scFv4C with and without KDEL tetrapeptide (scFv4CKDEL) were generated, as well as, three scFv2A versions: one cytosolic (scFv2A), one nuclear (NLS-scFv2A), and a cytosolic facing endoplasmic reticulum membrane targeted version (6K2-scFv2A). Transgenic Nicotiana benthamiana plants expressing these different scFv versions were obtained and some lines from each construct were selected for viral challenge. Challenge experiments were performed by mechanical inoculation with a PPV-D/NAT isolate at moderate inoculum doses (< 0.36 mug/ml viral concentration). The percentage of infected plants was significantly lower in some transgenic lines compared to control plants. Nuclear version of scFv2A and cytosolic version with KDEL of scFv4C showed the highest reduction in viral infection. |
| format | conferenceObject |
| id | ReDivia5282 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52822025-04-25T14:53:58Z Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection ACTA HORTICULTURAE Gil, Maite Esteban, Olga Martínez, M. Carmen Garcia, Juan Antonio Llácer, Gerardo Single chain variable fragments (scFv) specific to the coat protein (CP) (scFv4C) and the RNA replicase NIb (scFv2A) from Plum pox virus (PPV) were engineered. Diverse expression versions addressed to different cell compartments were produced by addition of several peptides. Two cytosolic versions of scFv4C with and without KDEL tetrapeptide (scFv4CKDEL) were generated, as well as, three scFv2A versions: one cytosolic (scFv2A), one nuclear (NLS-scFv2A), and a cytosolic facing endoplasmic reticulum membrane targeted version (6K2-scFv2A). Transgenic Nicotiana benthamiana plants expressing these different scFv versions were obtained and some lines from each construct were selected for viral challenge. Challenge experiments were performed by mechanical inoculation with a PPV-D/NAT isolate at moderate inoculum doses (< 0.36 mug/ml viral concentration). The percentage of infected plants was significantly lower in some transgenic lines compared to control plants. Nuclear version of scFv2A and cytosolic version with KDEL of scFv4C showed the highest reduction in viral infection. 2017-06-01T10:12:03Z 2017-06-01T10:12:03Z 2004 2004 conferenceObject Gil, M., Esteban, O., Martinez, M.C., Garcia, J.A. (2004). Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection. Proceedings of the Xixth International Symposium on Virus and Virus-Like Diseases of Temperate Fruit Crops: Fruit Tree Diseases, (657), 341-347. 0567-7572; 90-6605-148-5 http://hdl.handle.net/20.500.11939/5282 10.17660/ActaHortic.2004.657.54 en openAccess Impreso |
| spellingShingle | Gil, Maite Esteban, Olga Martínez, M. Carmen Garcia, Juan Antonio Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title | Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title_full | Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title_fullStr | Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title_full_unstemmed | Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title_short | Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral infection |
| title_sort | expression of single chain variable fragments against structural and non structural proteins of plum pox virus in transgenic nicotiana benthamiana plants to interfere with viral infection |
| url | http://hdl.handle.net/20.500.11939/5282 |
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