Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies
A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and s...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5208 |
| _version_ | 1855491885807697920 |
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| author | Forment, Javier Gadea, Jose Huerta, Laura Abizanda, L. Agustí, Javier Alamar, S. Alos, Enriqueta Andres, Fernando Arribas, R. Beltran, Jose P. Berbel, A. Blazquez, Miguel A. Brumos, Javier Canas, Luis A. Cercós, Manuel Colmenero-Flores, José M. Conesa, Ana Estables, B. Gandía, Mónica García-Martínez, José Luis Gimeno, Jacinta Gisbert, Ana D. Gomez, G. Gonzalez-Candelas, Luis Granell, Antonio Guerri, José Lafuente, M. T. Madueno, F. Marcos, J. F. Marques, M. C. Martinez, F. Martinez-Godoy, Maria A. Miralles, S. Moreno, Pedro Navarro, Luis Pallás, Vicente Pérez-Amador, Miguel A. Perez-Valle, Jorge Pons, C. Rodrigo, I. Rodriguez, P. L. Royo, C. Serrano, Ramón Soler, Guillermo Tadeo, Francisco R. Talón, Manuel Terol, Javier Trenor, M. Vaello, L. Vicente, Oscar Vidal, C. Zacarias, Lorenzo Conejero, Vicente |
| author_browse | Abizanda, L. Agustí, Javier Alamar, S. Alos, Enriqueta Andres, Fernando Arribas, R. Beltran, Jose P. Berbel, A. Blazquez, Miguel A. Brumos, Javier Canas, Luis A. Cercós, Manuel Colmenero-Flores, José M. Conejero, Vicente Conesa, Ana Estables, B. Forment, Javier Gadea, Jose Gandía, Mónica García-Martínez, José Luis Gimeno, Jacinta Gisbert, Ana D. Gomez, G. Gonzalez-Candelas, Luis Granell, Antonio Guerri, José Huerta, Laura Lafuente, M. T. Madueno, F. Marcos, J. F. Marques, M. C. Martinez, F. Martinez-Godoy, Maria A. Miralles, S. Moreno, Pedro Navarro, Luis Pallás, Vicente Perez-Valle, Jorge Pons, C. Pérez-Amador, Miguel A. Rodrigo, I. Rodriguez, P. L. Royo, C. Serrano, Ramón Soler, Guillermo Tadeo, Francisco R. Talón, Manuel Terol, Javier Trenor, M. Vaello, L. Vicente, Oscar Vidal, C. Zacarias, Lorenzo |
| author_facet | Forment, Javier Gadea, Jose Huerta, Laura Abizanda, L. Agustí, Javier Alamar, S. Alos, Enriqueta Andres, Fernando Arribas, R. Beltran, Jose P. Berbel, A. Blazquez, Miguel A. Brumos, Javier Canas, Luis A. Cercós, Manuel Colmenero-Flores, José M. Conesa, Ana Estables, B. Gandía, Mónica García-Martínez, José Luis Gimeno, Jacinta Gisbert, Ana D. Gomez, G. Gonzalez-Candelas, Luis Granell, Antonio Guerri, José Lafuente, M. T. Madueno, F. Marcos, J. F. Marques, M. C. Martinez, F. Martinez-Godoy, Maria A. Miralles, S. Moreno, Pedro Navarro, Luis Pallás, Vicente Pérez-Amador, Miguel A. Perez-Valle, Jorge Pons, C. Rodrigo, I. Rodriguez, P. L. Royo, C. Serrano, Ramón Soler, Guillermo Tadeo, Francisco R. Talón, Manuel Terol, Javier Trenor, M. Vaello, L. Vicente, Oscar Vidal, C. Zacarias, Lorenzo Conejero, Vicente |
| author_sort | Forment, Javier |
| collection | ReDivia |
| description | A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis. |
| format | Artículo |
| id | ReDivia5208 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia52082025-04-25T14:45:39Z Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies Forment, Javier Gadea, Jose Huerta, Laura Abizanda, L. Agustí, Javier Alamar, S. Alos, Enriqueta Andres, Fernando Arribas, R. Beltran, Jose P. Berbel, A. Blazquez, Miguel A. Brumos, Javier Canas, Luis A. Cercós, Manuel Colmenero-Flores, José M. Conesa, Ana Estables, B. Gandía, Mónica García-Martínez, José Luis Gimeno, Jacinta Gisbert, Ana D. Gomez, G. Gonzalez-Candelas, Luis Granell, Antonio Guerri, José Lafuente, M. T. Madueno, F. Marcos, J. F. Marques, M. C. Martinez, F. Martinez-Godoy, Maria A. Miralles, S. Moreno, Pedro Navarro, Luis Pallás, Vicente Pérez-Amador, Miguel A. Perez-Valle, Jorge Pons, C. Rodrigo, I. Rodriguez, P. L. Royo, C. Serrano, Ramón Soler, Guillermo Tadeo, Francisco R. Talón, Manuel Terol, Javier Trenor, M. Vaello, L. Vicente, Oscar Vidal, C. Zacarias, Lorenzo Conejero, Vicente A functional genomics project has been initiated to approach the molecular characterization of the main biological and agronomical traits of citrus. As a key part of this project, a citrus EST collection has been generated from 25 cDNA libraries covering different tissues, developmental stages and stress conditions. The collection includes a total of 22,635 high-quality ESTs, grouped in 11,836 putative unigenes, which represent at least one third of the estimated number of genes in the citrus genome. Functional annotation of unigenes which have Arabidopsis orthologues (68% of all unigenes) revealed gene representation in every major functional category, suggesting that a genome-wide EST collection was obtained. A Citrus clementina Hort. ex Tan. cv. Clemenules genomic library, that will contribute to further characterization of relevant genes, has also been constructed. To initiate the analysis of citrus transcriptome, we have developed a cDNA microarray containing 12,672 probes corresponding to 6875 putative unigenes of the collection. Technical characterization of the microarray showed high intra- and inter-array reproducibility, as well as a good range of sensitivity. We have also validated gene expression data achieved with this microarray through an independent technique such as RNA gel blot analysis. 2017-06-01T10:11:53Z 2017-06-01T10:11:53Z 2005 FEB article Forment, J., Gadea, J., Huerta, L., Abizanda, L., Agusti, J., Alamar, S., et al. (2005). Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies. Plant Molecular Biology, 57(3), 375-391. 0167-4412 http://hdl.handle.net/20.500.11939/5208 10.1007/s11103-004-7926-1 en openAccess Impreso |
| spellingShingle | Forment, Javier Gadea, Jose Huerta, Laura Abizanda, L. Agustí, Javier Alamar, S. Alos, Enriqueta Andres, Fernando Arribas, R. Beltran, Jose P. Berbel, A. Blazquez, Miguel A. Brumos, Javier Canas, Luis A. Cercós, Manuel Colmenero-Flores, José M. Conesa, Ana Estables, B. Gandía, Mónica García-Martínez, José Luis Gimeno, Jacinta Gisbert, Ana D. Gomez, G. Gonzalez-Candelas, Luis Granell, Antonio Guerri, José Lafuente, M. T. Madueno, F. Marcos, J. F. Marques, M. C. Martinez, F. Martinez-Godoy, Maria A. Miralles, S. Moreno, Pedro Navarro, Luis Pallás, Vicente Pérez-Amador, Miguel A. Perez-Valle, Jorge Pons, C. Rodrigo, I. Rodriguez, P. L. Royo, C. Serrano, Ramón Soler, Guillermo Tadeo, Francisco R. Talón, Manuel Terol, Javier Trenor, M. Vaello, L. Vicente, Oscar Vidal, C. Zacarias, Lorenzo Conejero, Vicente Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title | Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title_full | Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title_fullStr | Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title_full_unstemmed | Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title_short | Development of a citrus genome-wide EST collection and cDNA microarray as resources for genomic studies |
| title_sort | development of a citrus genome wide est collection and cdna microarray as resources for genomic studies |
| url | http://hdl.handle.net/20.500.11939/5208 |
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