Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance
Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citru...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.11939/5016 |
| _version_ | 1855032212634730496 |
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| author | Cervera, Magdalena Esteban, Olga Gil, Maite Gorris, María T. Martínez, M. Carmen Pena, Leandro Cambra, Mariano |
| author_browse | Cambra, Mariano Cervera, Magdalena Esteban, Olga Gil, Maite Gorris, María T. Martínez, M. Carmen Pena, Leandro |
| author_facet | Cervera, Magdalena Esteban, Olga Gil, Maite Gorris, María T. Martínez, M. Carmen Pena, Leandro Cambra, Mariano |
| author_sort | Cervera, Magdalena |
| collection | ReDivia |
| description | Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citrus could interfere and immunomodulate CTV infection, transgenic Mexican lime plants expressing two different scFv constructs, separately and simultaneously, were generated. These constructs derived from the well-referenced monoclonal antibodies 3DF1 and 3CA5, specific against CTV p25 major coat protein, whose mixture is able to detect all CTV isolates characterized so far. ScFv accumulation levels were low and could be readily detected just in four transgenic lines. Twelve homogeneous and vigorous lines were propagated and CTV-challenged by graft inoculation with an aggressive CTV strain. A clear protective effect was observed in most transgenic lines, which showed resistance in up to 40-60% of propagations. Besides, both a delay in symptom appearance and attenuation of symptom intensity were observed in infected transgenic plants compared with control plants. This effect was more evident in lines carrying the 3DF1scFv transgene, being probably related to the biological functions of the epitope recognized by this antibody. This is the first report describing successful protection against a pathogen in woody transgenic plants by ectopic expression of scFv recombinant antibodies. |
| format | article |
| id | ReDivia5016 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50162025-04-25T14:45:10Z Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance Cervera, Magdalena Esteban, Olga Gil, Maite Gorris, María T. Martínez, M. Carmen Pena, Leandro Cambra, Mariano Citrus tristeza virus (CTV) causes one of the most destructive viral diseases of citrus worldwide. Generation of resistant citrus genotypes through genetic engineering could be a good alternative to control CTV. To study whether production of single-chain variable fragment (scFv) antibodies in citrus could interfere and immunomodulate CTV infection, transgenic Mexican lime plants expressing two different scFv constructs, separately and simultaneously, were generated. These constructs derived from the well-referenced monoclonal antibodies 3DF1 and 3CA5, specific against CTV p25 major coat protein, whose mixture is able to detect all CTV isolates characterized so far. ScFv accumulation levels were low and could be readily detected just in four transgenic lines. Twelve homogeneous and vigorous lines were propagated and CTV-challenged by graft inoculation with an aggressive CTV strain. A clear protective effect was observed in most transgenic lines, which showed resistance in up to 40-60% of propagations. Besides, both a delay in symptom appearance and attenuation of symptom intensity were observed in infected transgenic plants compared with control plants. This effect was more evident in lines carrying the 3DF1scFv transgene, being probably related to the biological functions of the epitope recognized by this antibody. This is the first report describing successful protection against a pathogen in woody transgenic plants by ectopic expression of scFv recombinant antibodies. 2017-06-01T10:11:33Z 2017-06-01T10:11:33Z 2010 DEC 2010 article Cervera, M., Esteban, Olga, Gil, Maite, Teresa Gorris, M., C. Martinez, M., Pena, L., Cambra, M. (2010). Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance. Transgenic research, 19(6), 1001-1015. 0962-8819 http://hdl.handle.net/20.500.11939/5016 http://hdl.handle.net/20.500.11939/5016 10.1007/s11248-010-9378-5 en openAccess Impreso |
| spellingShingle | Cervera, Magdalena Esteban, Olga Gil, Maite Gorris, María T. Martínez, M. Carmen Pena, Leandro Cambra, Mariano Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title | Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title_full | Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title_fullStr | Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title_full_unstemmed | Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title_short | Transgenic expression in citrus of single-chain antibody fragments specific to Citrus tristeza virus confers virus resistance |
| title_sort | transgenic expression in citrus of single chain antibody fragments specific to citrus tristeza virus confers virus resistance |
| url | http://hdl.handle.net/20.500.11939/5016 http://hdl.handle.net/20.500.11939/5016 |
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