Use of Specific Monoclonal Antibodies for Diagnosis of Citrus Tristeza Virus
Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained by fusion of a nonsecreting myeloma cell line with spleen cells from immunized BALB/c mice. Immunoglobulin titre and isotype were determined in cell culture supernatants and mouse ascitic fluid. Each MAb was enzyme-co...
| Autores principales: | , , , |
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| Formato: | conferenceObject |
| Lenguaje: | Inglés |
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IOCV
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.11939/7694 https://iocv.ucr.edu/sites/g/files/rcwecm4696/files/2020-06/10th055_061.pdf |
| Sumario: | Monoclonal antibodies (MAb) specific for citrus tristeza virus (CTV) were obtained by fusion of a nonsecreting myeloma cell line with spleen cells from immunized BALB/c mice. Immunoglobulin titre and isotype were determined in cell culture supernatants and mouse ascitic fluid. Each MAb was enzyme-conjugated and used in a double-antibody sandwich ELISA assay for CTV. All reacted uniformly against a panel of 23 strains of CTV representative of strains collected from Europe, Asia, and North America. |
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