A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype
A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5013 |
| _version_ | 1855491851995316224 |
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| author | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro |
| author_browse | Cervera, Magdalena Juárez, José Navarro, Luis Pena, Leandro Pina, José A. |
| author_facet | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro |
| author_sort | Cervera, Magdalena |
| collection | ReDivia |
| description | A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus. |
| format | Artículo |
| id | ReDivia5013 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia50132025-04-25T14:45:09Z A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus. 2017-06-01T10:11:33Z 2017-06-01T10:11:33Z 2000 MAR article Cervera, M., Pina, JA, Juarez, J., Navarro, L., Pena, L. (2000). A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype. Theoretical and Applied Genetics, 100(5), 670-677. 0040-5752 http://hdl.handle.net/20.500.11939/5013 10.1007/s001220051338 en openAccess Impreso |
| spellingShingle | Cervera, Magdalena Pina, José A. Juárez, José Navarro, Luis Pena, Leandro A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title | A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title_full | A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title_fullStr | A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title_full_unstemmed | A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title_short | A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype |
| title_sort | broad exploration of a transgenic population of citrus stability of gene expression and phenotype |
| url | http://hdl.handle.net/20.500.11939/5013 |
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