A broad exploration of a transgenic population of citrus: stability of gene expression and phenotype
A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/5013 |
| Sumario: | A collection of 70 transgenic citrus plants for the uidA and nptII genes have been maintained under screenhouse conditions over a period of 4-5 years. A detailed scanning of the plants allowed us to detect four phenotypic off-type plants and a large variation of transgene integration and expression patterns among the population. Off-type plants were analysed and characterised as nucellar tetraploids, probably originating from tetraploid starting tissues rather than from somaclonal variation events. Transgene integration and expression analyses revealed that: (I) a significant negative correlation was found between copy number and GUS activity; (2) rearrangements of the T-DNA inserts did not imply low expression levels; and (3) stability of integration and expression of the transgenes was confirmed for all the transformants grown under natural environmental conditions. These combined features validate transformation as a tool for the genetic improvement of citrus. |
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