Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin
Egg yolk (BY) and glycerol are common constituents of extenders used for sperm cryopreservation. It has been demonstrated that using cholesterol-loaded cyclodextrins (CLC) improves sperm cryosurvival in several species. However, standard freezing extenders might not be the most appropriate for CLC-t...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4854 |
| _version_ | 1855032187735244800 |
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| author | Blanch, Eva Tomás, Cristina Hernandez, Marta Roca, Jordi Martinez, Emilio A. Vazquez, Juan M. Mocé, Eva |
| author_browse | Blanch, Eva Hernandez, Marta Martinez, Emilio A. Mocé, Eva Roca, Jordi Tomás, Cristina Vazquez, Juan M. |
| author_facet | Blanch, Eva Tomás, Cristina Hernandez, Marta Roca, Jordi Martinez, Emilio A. Vazquez, Juan M. Mocé, Eva |
| author_sort | Blanch, Eva |
| collection | ReDivia |
| description | Egg yolk (BY) and glycerol are common constituents of extenders used for sperm cryopreservation. It has been demonstrated that using cholesterol-loaded cyclodextrins (CLC) improves sperm cryosurvival in several species. However, standard freezing extenders might not be the most appropriate for CLC-treated sperm. This study evaluated the EY and glycerol requirements for freezing. CLC-treated boar spermatozoa. Semen samples from 34 ejaculates coming from 4 boars were used. Each ejaculate was split into three aliquots: one was used untreated (control), and the other two were treated with 1 mg of CLC or methyl-beta-cyclodextrin/120 x 10(6) sperm for 15 min at 22 C prior to cryopreservation. Our results indicated that reducing the concentration of EY was detrimental for sperm viability after thawing (31.57 +/- 2 vs. 19.89% +/- 2 for 20 and 10% EY, respectively; P < 0.05), even in semen treated with CLC. On the other hand, it was observed that the traditional concentration of Glycerol (3%) was not the appropriate for freezing CLC-treated sperm (61.10 +/- 3 vs. 47.87% +/- 3 viable sperm for control and CLC-treated sperm, respectively; P <0.05). Thus, CLC-treated sperm showed a higher tolerance to high glycerol concentrations (5%) in terms of sperm viability (59.19% +/- 3) than non-treated sperm (45.58% +/- 3; P<0.05). Therefore, it could be necessary to modify the freeling extenders for CLC-treated sperm. Nevertheless, additional studies will be needed to evaluate alternative cryoprotectants and to determine the effect of high glycerol concentrations on sperm functionality. |
| format | article |
| id | ReDivia4854 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia48542025-04-25T14:44:47Z Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin Blanch, Eva Tomás, Cristina Hernandez, Marta Roca, Jordi Martinez, Emilio A. Vazquez, Juan M. Mocé, Eva Egg yolk (BY) and glycerol are common constituents of extenders used for sperm cryopreservation. It has been demonstrated that using cholesterol-loaded cyclodextrins (CLC) improves sperm cryosurvival in several species. However, standard freezing extenders might not be the most appropriate for CLC-treated sperm. This study evaluated the EY and glycerol requirements for freezing. CLC-treated boar spermatozoa. Semen samples from 34 ejaculates coming from 4 boars were used. Each ejaculate was split into three aliquots: one was used untreated (control), and the other two were treated with 1 mg of CLC or methyl-beta-cyclodextrin/120 x 10(6) sperm for 15 min at 22 C prior to cryopreservation. Our results indicated that reducing the concentration of EY was detrimental for sperm viability after thawing (31.57 +/- 2 vs. 19.89% +/- 2 for 20 and 10% EY, respectively; P < 0.05), even in semen treated with CLC. On the other hand, it was observed that the traditional concentration of Glycerol (3%) was not the appropriate for freezing CLC-treated sperm (61.10 +/- 3 vs. 47.87% +/- 3 viable sperm for control and CLC-treated sperm, respectively; P <0.05). Thus, CLC-treated sperm showed a higher tolerance to high glycerol concentrations (5%) in terms of sperm viability (59.19% +/- 3) than non-treated sperm (45.58% +/- 3; P<0.05). Therefore, it could be necessary to modify the freeling extenders for CLC-treated sperm. Nevertheless, additional studies will be needed to evaluate alternative cryoprotectants and to determine the effect of high glycerol concentrations on sperm functionality. 2017-06-01T10:11:11Z 2017-06-01T10:11:11Z 2014 APR 2014 article Blanch, Eva, Tomas, C., Hernandez, Marta, Roca, Jordi, Martinez, Emilio A., Vazquez, J. M., Moce, E. (2014). Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or CholE.ol-loaded Cyclodextrin. Journal of Reproduction and Development, 60(2), 143-149. 0916-8818 http://hdl.handle.net/20.500.11939/4854 10.1262/jrd.2013-073 en openAccess Impreso |
| spellingShingle | Blanch, Eva Tomás, Cristina Hernandez, Marta Roca, Jordi Martinez, Emilio A. Vazquez, Juan M. Mocé, Eva Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title | Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title_full | Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title_fullStr | Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title_full_unstemmed | Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title_short | Egg Yolk and Glycerol Requirements for Freezing Boar Spermatozoa Treated with Methyl beta-Cyclodextrin or Cholesterol-loaded Cyclodextrin |
| title_sort | egg yolk and glycerol requirements for freezing boar spermatozoa treated with methyl beta cyclodextrin or cholesterol loaded cyclodextrin |
| url | http://hdl.handle.net/20.500.11939/4854 |
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