In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen
The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, it? vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4762 |
| _version_ | 1855491809642283008 |
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| author | Viudes-De-Castro, María P. Mocé, Eva Vicente, José Salvador Marco-Jiménez, Francisco Lavara, R. |
| author_browse | Lavara, R. Marco-Jiménez, Francisco Mocé, Eva Vicente, José Salvador Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Mocé, Eva Vicente, José Salvador Marco-Jiménez, Francisco Lavara, R. |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, it? vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated with frozen-thawed spermatozoa. Oocytes used for in vitro testing were recovered 14 h after ovulation induction from donors and co-incubated with 2 x 10(6) frozen-thawed spermatozoa during 4 h at 37 degrees C in Tyrode's medium under an atmosphere of 5% CO(2) in air with maximal humidity. After co-incubation period, presumptive zygotes were cultured in TCM199 supplemented with 20% foetal bovine serum (FBS), under the same conditions described above. Although no statistical differences were observed between freezing protocols in seminal parameters [motility rate: 40 and 35%, VCL 35 and 46 mu m/s, amplitude of lateral head displacement (ALH): 1.7 and 2.4 mu m, for semen frozen at -30 degrees C and in LNV, respectively], significant differences were noted in the fertilizing ability in vivo and in vitro. Semen frozen at -30 degrees C showed the highest fertilizing ability in vitro (26.7% vs 6.2 and 8.7% for semen frozen at -30 degrees C, in LNV and fresh semen, respectively) and the lowest fertility rate in vivo (21.7% vs 64.2% and 70.6% for semen frozen at -30 degrees C, in LNV and fresh semen, respectively). Sperm frozen at -30 degrees C seemed to be more capacitated. |
| format | Artículo |
| id | ReDivia4762 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia47622025-04-25T14:44:20Z In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen Viudes-De-Castro, María P. Mocé, Eva Vicente, José Salvador Marco-Jiménez, Francisco Lavara, R. The present work studied different spermatozoa parameters and the ability of frozen rabbit spermatozoa to fertilize, it? vitro, in vivo-matured oocytes, as a test to predict their in vivo fertility and prolificacy. Semen from rabbit bucks was frozen using two freezing protocols [in a freezer at -30 degrees C or in liquid nitrogen vapour (LNV)]. For the in vivo trial, females were inseminated with frozen-thawed spermatozoa. Oocytes used for in vitro testing were recovered 14 h after ovulation induction from donors and co-incubated with 2 x 10(6) frozen-thawed spermatozoa during 4 h at 37 degrees C in Tyrode's medium under an atmosphere of 5% CO(2) in air with maximal humidity. After co-incubation period, presumptive zygotes were cultured in TCM199 supplemented with 20% foetal bovine serum (FBS), under the same conditions described above. Although no statistical differences were observed between freezing protocols in seminal parameters [motility rate: 40 and 35%, VCL 35 and 46 mu m/s, amplitude of lateral head displacement (ALH): 1.7 and 2.4 mu m, for semen frozen at -30 degrees C and in LNV, respectively], significant differences were noted in the fertilizing ability in vivo and in vitro. Semen frozen at -30 degrees C showed the highest fertilizing ability in vitro (26.7% vs 6.2 and 8.7% for semen frozen at -30 degrees C, in LNV and fresh semen, respectively) and the lowest fertility rate in vivo (21.7% vs 64.2% and 70.6% for semen frozen at -30 degrees C, in LNV and fresh semen, respectively). Sperm frozen at -30 degrees C seemed to be more capacitated. 2017-06-01T10:10:56Z 2017-06-01T10:10:56Z 2005 APR 2005 article Viudes-De-Castro, M.P., Moce, E., Vicente, J.S., Marco-Jimenez, F., Lavara, R. (2005). In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen. Reproduction in Domestic Animals, 40(2), 136-140. 0936-6768 http://hdl.handle.net/20.500.11939/4762 10.1111/j.1439-0531.2005.00568.x en openAccess Impreso |
| spellingShingle | Viudes-De-Castro, María P. Mocé, Eva Vicente, José Salvador Marco-Jiménez, Francisco Lavara, R. In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title | In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title_full | In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title_fullStr | In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title_full_unstemmed | In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title_short | In vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| title_sort | in vitro evaluation of in vivo fertilizing ability of frozen rabbit semen |
| url | http://hdl.handle.net/20.500.11939/4762 |
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