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A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs

Areawide sterile insect technique (SIT) programs against Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), are increasingly implemented worldwide. A key issue in SIT is to assess mating success of released sterile males, which could be currently estimated by egg hatchab...

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Main Authors: San-Andrés, Victoria, Urbaneja, Alberto, Sabater-Munoz, Beatriz, Castanera, Pedro
Format: Artículo
Language:Inglés
Published: 2017
Subjects:
Animals
Biological Markers/analysis
Classification/methods
Female
Male
Pest Control, Biological
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Sexual Behavior, Animal
Spermatozoa/classification
Tephritidae/genetics/physiology
Online Access:http://hdl.handle.net/20.500.11939/4482
https://academic.oup.com/jee/article-abstract/100/4/1444/2198895?redirectedFrom=fulltext
Summary:Areawide sterile insect technique (SIT) programs against Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), are increasingly implemented worldwide. A key issue in SIT is to assess mating success of released sterile males, which could be currently estimated by egg hatchability and by stored sperm head measurements. We report here on a novel molecular approach that would allow detecting the presence of Mediterranean fruit fly sterile male sperm in the female spermathecae under field conditions, as a precise marker to assess mating performance. The simplicity (only two polymerase chain reactions) and reliability of this method, jointly with the capability to detect Vienna sperm in wild Mediterranean fruit fly maintained in monitoring traps for 7 d under field conditions, suggest that it could be an efficient tool when coupled with areawide SIT programs.

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