A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs
Areawide sterile insect technique (SIT) programs against Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), are increasingly implemented worldwide. A key issue in SIT is to assess mating success of released sterile males, which could be currently estimated by egg hatchab...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4482 https://academic.oup.com/jee/article-abstract/100/4/1444/2198895?redirectedFrom=fulltext |
| _version_ | 1855491761806245888 |
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| author | San-Andrés, Victoria Urbaneja, Alberto Sabater-Munoz, Beatriz Castanera, Pedro |
| author_browse | Castanera, Pedro Sabater-Munoz, Beatriz San-Andrés, Victoria Urbaneja, Alberto |
| author_facet | San-Andrés, Victoria Urbaneja, Alberto Sabater-Munoz, Beatriz Castanera, Pedro |
| author_sort | San-Andrés, Victoria |
| collection | ReDivia |
| description | Areawide sterile insect technique (SIT) programs against Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), are increasingly implemented worldwide. A key issue in SIT is to assess mating success of released sterile males, which could be currently estimated by egg hatchability and by stored sperm head measurements. We report here on a novel molecular approach that would allow detecting the presence of Mediterranean fruit fly sterile male sperm in the female spermathecae under field conditions, as a precise marker to assess mating performance. The simplicity (only two polymerase chain reactions) and reliability of this method, jointly with the capability to detect Vienna sperm in wild Mediterranean fruit fly maintained in monitoring traps for 7 d under field conditions, suggest that it could be an efficient tool when coupled with areawide SIT programs. |
| format | Artículo |
| id | ReDivia4482 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia44822025-04-25T14:43:39Z A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs San-Andrés, Victoria Urbaneja, Alberto Sabater-Munoz, Beatriz Castanera, Pedro Animals Biological Markers/analysis Classification/methods Female Male Pest Control, Biological Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sexual Behavior, Animal Spermatozoa/classification Tephritidae/genetics/physiology Areawide sterile insect technique (SIT) programs against Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), are increasingly implemented worldwide. A key issue in SIT is to assess mating success of released sterile males, which could be currently estimated by egg hatchability and by stored sperm head measurements. We report here on a novel molecular approach that would allow detecting the presence of Mediterranean fruit fly sterile male sperm in the female spermathecae under field conditions, as a precise marker to assess mating performance. The simplicity (only two polymerase chain reactions) and reliability of this method, jointly with the capability to detect Vienna sperm in wild Mediterranean fruit fly maintained in monitoring traps for 7 d under field conditions, suggest that it could be an efficient tool when coupled with areawide SIT programs. 2017-06-01T10:10:14Z 2017-06-01T10:10:14Z 2007 Aug article San Andres, V., Urbaneja, A., Sabater-Munoz, B., Castanera, P. (2007). A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae), males in sterile insect technique programs. Journal of economic entomology, 100(4), 1444-1449. 0022-0493; 0022-0493 http://hdl.handle.net/20.500.11939/4482 10.1093/jee/100.4.1444 https://academic.oup.com/jee/article-abstract/100/4/1444/2198895?redirectedFrom=fulltext en openAccess Impreso |
| spellingShingle | Animals Biological Markers/analysis Classification/methods Female Male Pest Control, Biological Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sexual Behavior, Animal Spermatozoa/classification Tephritidae/genetics/physiology San-Andrés, Victoria Urbaneja, Alberto Sabater-Munoz, Beatriz Castanera, Pedro A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title | A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title_full | A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title_fullStr | A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title_full_unstemmed | A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title_short | A novel molecular approach to assess mating success of sterile Ceratitis capitata (Diptera: Tephritidae) males in sterile insect technique programs |
| title_sort | novel molecular approach to assess mating success of sterile ceratitis capitata diptera tephritidae males in sterile insect technique programs |
| topic | Animals Biological Markers/analysis Classification/methods Female Male Pest Control, Biological Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Sexual Behavior, Animal Spermatozoa/classification Tephritidae/genetics/physiology |
| url | http://hdl.handle.net/20.500.11939/4482 https://academic.oup.com/jee/article-abstract/100/4/1444/2198895?redirectedFrom=fulltext |
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