A fast one-step reverse transcription and polymerase chain reaction (RT-PCR) amplification procedure providing highly specific complementary DNA from plant virus RNA

Reverse transcription and polymerase chain reaction (RT-PCR) are being used increasingly for detection and typing RNA viruses. For this purpose, metal block thermal cyclers (MBTC) are considered to provide higher DNA yield, whereas air thermal cyclers (ATC) allow PCR amplification in a much shorter...

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Bibliographic Details
Main Authors: Sambade, A., Martin, Susana, Olmos, Antonio, Garcia, M. L., Cambra, Mariano, Grau, Oscar, Guerri, José, Moreno, Pedro
Format: Artículo
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4478

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