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Chromogenic detection of yam mosaic virus by closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP)

A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the...

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Main Authors: Nkere, C.K., Oyekanmi, J.O., Silva, G., Bömer, M., Atiri, G.I., Onyeka, J., Maroya, N.G., Seal, S.E., Kumar, P. Lava
Format: Journal Article
Language:Inglés
Published: Springer 2018
Subjects:
yams
viruses
plant viruses
plant condition
plant diseases
production
virology
Online Access:https://hdl.handle.net/10568/108663
Summary:A closed-tube reverse transcription loop-mediated isothermal amplification (CT-RT-LAMP) assay was developed for the detection of yam mosaic virus (YMV, genus Potyvirus) infecting yam (Dioscorea spp.). The assay uses a set of six oligonucleotide primers targeting the YMV coat protein region, and the amplification products in YMV-positive samples are visualized by chromogenic detection with SYBR Green I dye. The CT-RT-LAMP assay detected YMV in leaf and tuber tissues of infected plants. The assay is 100 times more sensitive in detecting YMV than standard RT-PCR, while maintaining the same specificity.

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