Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties
Orange is a major crop and an important source of health-promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the -carotene content of ora...
| Autores principales: | , , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4377 |
| _version_ | 1855032110385987584 |
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| author | Pons, Elsa Alquezar, Berta Rodriguez, Ana Martorell, Patricia Genoves, Salvador Ramon, Daniel Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro |
| author_browse | Alquezar, Berta Genoves, Salvador Martorell, Patricia Pena, Leandro Pons, Elsa Ramon, Daniel Rodrigo, María J. Rodriguez, Ana Zacarias, Lorenzo |
| author_facet | Pons, Elsa Alquezar, Berta Rodriguez, Ana Martorell, Patricia Genoves, Salvador Ramon, Daniel Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro |
| author_sort | Pons, Elsa |
| collection | ReDivia |
| description | Orange is a major crop and an important source of health-promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the -carotene content of orange fruit through blocking by RNA interference the expression of an endogenous -carotene hydroxylase gene (Cs-CHX) that is involved in the conversion of -carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the FLOWERING LOCUS T from sweet orange (CsFT), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the Cs-CHX gene resulted in oranges with a deep yellow (golden') phenotype and significant increases (up to 36-fold) in -carotene content in the pulp. The capacity of -carotene-enriched oranges for protection against oxidative stress in vivo was assessed using Caenorhabditis elegans as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the -carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops. |
| format | article |
| id | ReDivia4377 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43772025-04-25T14:42:59Z Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties Pons, Elsa Alquezar, Berta Rodriguez, Ana Martorell, Patricia Genoves, Salvador Ramon, Daniel Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro Orange is a major crop and an important source of health-promoting bioactive compounds. Increasing the levels of specific antioxidants in orange fruit through metabolic engineering could strengthen the fruit's health benefits. In this work, we have afforded enhancing the -carotene content of orange fruit through blocking by RNA interference the expression of an endogenous -carotene hydroxylase gene (Cs-CHX) that is involved in the conversion of -carotene into xanthophylls. Additionally, we have simultaneously overexpressed a key regulator gene of flowering transition, the FLOWERING LOCUS T from sweet orange (CsFT), in the transgenic juvenile plants, which allowed us to obtain fruit in an extremely short period of time. Silencing the Cs-CHX gene resulted in oranges with a deep yellow (golden') phenotype and significant increases (up to 36-fold) in -carotene content in the pulp. The capacity of -carotene-enriched oranges for protection against oxidative stress in vivo was assessed using Caenorhabditis elegans as experimental animal model. Golden oranges induced a 20% higher antioxidant effect than the isogenic control. This is the first example of the successful metabolic engineering of the -carotene content (or the content of any other phytonutrient) in oranges and demonstrates the potential of genetic engineering for the nutritional enhancement of fruit tree crops. 2017-06-01T10:09:58Z 2017-06-01T10:09:58Z 2014 JAN 2014 article Pons, E., Alquezar, B., Rodriguez, A., Martorell, P., Genoves, Salvador, R., Daniel, Jesus Rodrigo, M., Zacarias, L., Pena, L. (2014). Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties. Plant Biotechnology Journal, 12(1), 17-27. 1467-7644 http://hdl.handle.net/20.500.11939/4377 10.1111/pbi.12112 en openAccess Impreso |
| spellingShingle | Pons, Elsa Alquezar, Berta Rodriguez, Ana Martorell, Patricia Genoves, Salvador Ramon, Daniel Rodrigo, María J. Zacarias, Lorenzo Pena, Leandro Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title | Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title_full | Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title_fullStr | Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title_full_unstemmed | Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title_short | Metabolic engineering of beta-carotene in orange fruit increases its in vivo antioxidant properties |
| title_sort | metabolic engineering of beta carotene in orange fruit increases its in vivo antioxidant properties |
| url | http://hdl.handle.net/20.500.11939/4377 |
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