Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time
Citrus trees have a long juvenile phase that delays their reproductive development by between 6 and 20 years, depending on the species. With the aim of accelerating their flowering time, we transformed juvenile citrus seedlings to constitutively express the Arabidopsis LEAFY(LFY) or APETALA1 (AP1) g...
| Main Authors: | , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.11939/4336 |
| _version_ | 1855491735091675136 |
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| author | Pena, Leandro Martin-Trillo, M. Juárez, José Pina, José A. Navarro, Luis Martinez-Zapater, JM |
| author_browse | Juárez, José Martin-Trillo, M. Martinez-Zapater, JM Navarro, Luis Pena, Leandro Pina, José A. |
| author_facet | Pena, Leandro Martin-Trillo, M. Juárez, José Pina, José A. Navarro, Luis Martinez-Zapater, JM |
| author_sort | Pena, Leandro |
| collection | ReDivia |
| description | Citrus trees have a long juvenile phase that delays their reproductive development by between 6 and 20 years, depending on the species. With the aim of accelerating their flowering time, we transformed juvenile citrus seedlings to constitutively express the Arabidopsis LEAFY(LFY) or APETALA1 (AP1) genes, which promote flower initiation in Arabidopsis. Both types of transgenic citrus produced fertile flowers and fruits as early as the first year, notably through a mechanism involving an appreciable shortening of their juvenile phase. Furthermore, expression of AP1 was as efficient as LFY in the initiation of flowers, and did not produce any severe developmental abnormality. Both types of transgenic trees flowered in consecutive years, and their flowering response was under environmental control. In addition, zygotic and nucellar derived transgenic seedlings had a very short juvenile phase and flowered in their first spring, demonstrating the stability and inheritance of this trait. These results open new possibilities for domestication, genetic improvement, and experimental research in citrus and other woody species. |
| format | Artículo |
| id | ReDivia4336 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia43362025-04-25T14:42:53Z Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time Pena, Leandro Martin-Trillo, M. Juárez, José Pina, José A. Navarro, Luis Martinez-Zapater, JM Citrus trees have a long juvenile phase that delays their reproductive development by between 6 and 20 years, depending on the species. With the aim of accelerating their flowering time, we transformed juvenile citrus seedlings to constitutively express the Arabidopsis LEAFY(LFY) or APETALA1 (AP1) genes, which promote flower initiation in Arabidopsis. Both types of transgenic citrus produced fertile flowers and fruits as early as the first year, notably through a mechanism involving an appreciable shortening of their juvenile phase. Furthermore, expression of AP1 was as efficient as LFY in the initiation of flowers, and did not produce any severe developmental abnormality. Both types of transgenic trees flowered in consecutive years, and their flowering response was under environmental control. In addition, zygotic and nucellar derived transgenic seedlings had a very short juvenile phase and flowered in their first spring, demonstrating the stability and inheritance of this trait. These results open new possibilities for domestication, genetic improvement, and experimental research in citrus and other woody species. 2017-06-01T10:09:52Z 2017-06-01T10:09:52Z 2001 MAR article Pena, L., Martin-Trillo, M., Juarez, J., Pina, JA, Navarro, L., Martinez-Zapater, JM (2001). Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time. Nature biotechnology, 19(3), 263-267. 1087-0156 http://hdl.handle.net/20.500.11939/4336 10.1038/85719 en openAccess Impreso |
| spellingShingle | Pena, Leandro Martin-Trillo, M. Juárez, José Pina, José A. Navarro, Luis Martinez-Zapater, JM Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title | Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title_full | Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title_fullStr | Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title_full_unstemmed | Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title_short | Constitutive expression of Arabidopsis LEAFY or APETALA1 genes in citrus reduces their generation time |
| title_sort | constitutive expression of arabidopsis leafy or apetala1 genes in citrus reduces their generation time |
| url | http://hdl.handle.net/20.500.11939/4336 |
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