Real-time RT-PCR for quantitative detection of Plum pox virus

Two protocols for real-time quantitative detection of Plum pox virus (PPV) and identification of PPV-D and PPV-M isolates were developed using the intercalating dye SYBR-Green I and TaqMan chemistry. SYBR-Green I method using the universal PPV primers, P1 and P2, required for its optimization an acc...

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Bibliographic Details
Main Authors:	Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano
Other Authors:	Llácer, Gerardo
Format:	conferenceObject
Language:	Inglés
Published:	2017
Online Access:	http://hdl.handle.net/20.500.11939/4257

Characterization and detection of plum pox virus using monoclonal antibodies by Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

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Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA by Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

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Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

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European protocol for diagnosis of Erwinia amylovora by López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

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Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral in... by Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio

Published 2017

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Fire blight in Spain: Situation and monitoring by López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

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Isothermal amplification for detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Published 2017

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Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction by Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

Published 2017

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Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction by Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

Published 2017

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Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union by López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

Published 2017

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Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas by Cambra, Mariano

Published 2017

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Thirteen years of sharka disease in Valencia, Spain by Llácer, Gerardo, Cambra, Mariano

Published 2017

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conferenceObject

Real-time RT-PCR for quantitative detection of Plum pox virus

Characterization and detection of plum pox virus using monoclonal antibodies by Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA by Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options by Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Epidemiology of Plum pox virus in Japanese plums in Spain by Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

European protocol for diagnosis of Erwinia amylovora by López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral in... by Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio

Fire blight in Spain: Situation and monitoring by López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

Isothermal amplification for detection of Plum pox virus by Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction by Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction by Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union by López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas by Cambra, Mariano

Thirteen years of sharka disease in Valencia, Spain by Llácer, Gerardo, Cambra, Mariano

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