Real-time RT-PCR for quantitative detection of Plum pox virus

Two protocols for real-time quantitative detection of Plum pox virus (PPV) and identification of PPV-D and PPV-M isolates were developed using the intercalating dye SYBR-Green I and TaqMan chemistry. SYBR-Green I method using the universal PPV primers, P1 and P2, required for its optimization an acc...

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Detalles Bibliográficos
Autores principales:	Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano
Otros Autores:	Llácer, Gerardo
Formato:	conferenceObject
Lenguaje:	Inglés
Publicado:	2017
Acceso en línea:	http://hdl.handle.net/20.500.11939/4257

Characterization and detection of plum pox virus using monoclonal antibodies por Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

Publicado 2017

Enlace del recurso

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Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA por Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

Publicado 2017

Enlace del recurso

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Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options por Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Publicado 2017

Enlace del recurso

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Epidemiology of Plum pox virus in Japanese plums in Spain por Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

Publicado 2017

Enlace del recurso

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European protocol for diagnosis of Erwinia amylovora por López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

Publicado 2017

Enlace del recurso

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Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral in... por Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio

Publicado 2017

Enlace del recurso

conferenceObject

Fire blight in Spain: Situation and monitoring por López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

Publicado 2017

Enlace del recurso

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Isothermal amplification for detection of Plum pox virus por Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Publicado 2017

Enlace del recurso

conferenceObject

Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction por Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

Publicado 2017

Enlace del recurso
Enlace del recurso

conferenceObject

Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction por Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

Publicado 2017

Enlace del recurso

conferenceObject

Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union por López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

Publicado 2017

Enlace del recurso

conferenceObject

Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas por Cambra, Mariano

Publicado 2017

Enlace del recurso

conferenceObject

Thirteen years of sharka disease in Valencia, Spain por Llácer, Gerardo, Cambra, Mariano

Publicado 2017

Enlace del recurso

conferenceObject

Real-time RT-PCR for quantitative detection of Plum pox virus

Characterization and detection of plum pox virus using monoclonal antibodies por Asensio, M., Gorris, María T., Sanz, Antonio, Camarasa, E., Perez, E., Carbonell, Emilio A., Cambra, Mariano

Detection and typing of prunus viruses in plant tissues and in vectors by print and spot-capture PCR, heminested-PCR and PCR-ELISA por Cambra, Mariano, Olmos, Antonio, Asensio, M., Esteban, Olga, Gorris, María T., Candresse, Thierry, Boscia, D.

Diagnosis and Detection of Plum Pox Virus: State-of-the-Art and Future Options por Cambra, Mariano, Bertolini, Edson, Martínez, M. Carmen, Gorris, María T., Vidal, Eduardo, Olmos, Antonio

Epidemiology of Plum pox virus in Japanese plums in Spain por Cambra, Mariano, Gorris, María T., Capote, Nieves, Asensio, M., Martínez, M. Carmen, Bertolini, Edson, Collado, C., Hermoso-De-Mendoza, Alfonso, Mataix-Gato, Emilio, Lopez, A.

European protocol for diagnosis of Erwinia amylovora por López, María M., Llop, Pablo, Gorris, María T., Penyalver, Javier, Donat, Victoria, Cambra, Mariano, Keck, M.

Expression of single-chain variable fragments against structural and non-structural proteins of Plum pox virus in transgenic Nicotiana benthamiana plants to interfere with viral in... por Gil, Maite, Esteban, Olga, Martínez, M. Carmen, Garcia, Juan Antonio

Fire blight in Spain: Situation and monitoring por López, María M., Gorris, María T., Llop, Pablo, Cambra, Mariano, Roselló, Montserrat, Berra, D., Borruel, M., Plaza, B., Garcia, P., Palomo, José L.

Isothermal amplification for detection of Plum pox virus por Olmos, Antonio, Bertolini, Edson, Cambra, Mariano

Quantitative trait loci and candidate gene analysis of citrus tristeza virus-citradia interaction por Asins, María J., Bernet, Guillermo P., Ruiz, C., Cambra, Mariano, Puchades, Juan, Carbonell, Emilio A.

Spot real-time RT-PCR: A method for direct detection of Plum pox virus avoiding RNA extraction por Capote, Nieves, Bertolini, Edson, Martínez, M. Carmen, Olmos, Antonio, Gorris, María T., Cambra, Mariano

Standardization of diagnostic protocols (Diagpro) for Erwinia amylovora in the European Union por López, María M., Gorris, María T., Llop, Pablo, Penyalver, Javier, Cambra, Mariano

Strategies for the control of Plum Pox Virus in apricot in Mediterranean areas por Cambra, Mariano

Thirteen years of sharka disease in Valencia, Spain por Llácer, Gerardo, Cambra, Mariano

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