Real-time RT-PCR for quantitative detection of Plum pox virus

Two protocols for real-time quantitative detection of Plum pox virus (PPV) and identification of PPV-D and PPV-M isolates were developed using the intercalating dye SYBR-Green I and TaqMan chemistry. SYBR-Green I method using the universal PPV primers, P1 and P2, required for its optimization an acc...

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Bibliographic Details
Main Authors: Olmos, Antonio, Bertolini, Edson, Gil, Maite, Cambra, Mariano
Other Authors: Llácer, Gerardo
Format: conferenceObject
Language:Inglés
Published: 2017
Online Access:http://hdl.handle.net/20.500.11939/4257

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