Deastringency treatment with CO2 induces oxidative stress in persimmon fruit

Because of astringency at harvest, 'Rojo Brillante' persimmons are regularly submitted to deastringency treatment based on exposing fruit to a high CO2 concentration. The treatment conditions that ensure total astringency removal throughout the various maturity stages have been determined to...

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Autores principales: Novillo, Pedro, Salvador, Alejandra, Magalhaes, Tahise, Besada, Cristina
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Acceso en línea:http://hdl.handle.net/20.500.11939/4222
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author Novillo, Pedro
Salvador, Alejandra
Magalhaes, Tahise
Besada, Cristina
author_browse Besada, Cristina
Magalhaes, Tahise
Novillo, Pedro
Salvador, Alejandra
author_facet Novillo, Pedro
Salvador, Alejandra
Magalhaes, Tahise
Besada, Cristina
author_sort Novillo, Pedro
collection ReDivia
description Because of astringency at harvest, 'Rojo Brillante' persimmons are regularly submitted to deastringency treatment based on exposing fruit to a high CO2 concentration. The treatment conditions that ensure total astringency removal throughout the various maturity stages have been determined to be 95% CO2, 20 degrees C, 24 h. The aim of this study was to investigate changes in the redox state of persimmon fruit associated with this deastringency treatment. The levels of reactive oxygen species (ROS) (O-2(-) and H2O2), and the activities of the main ROS scavenging enzymes (CAT, POD, APX, and SOD), were determined at harvest and after deastringency in fruit at three different maturity stages. Our results showed that during 'Rojo Brillante' persimmon maturation, the level of O-2(-) gradually increased, while APX activity was lowered. The deastringency treatment with CO2 induced oxidative stress in the fruit, observed as an over-accumulation of O-2(-) and H2O2. As a response to ROS accumulation, the activities of the CAT, APX and SOD scavenging enzymes were up-regulated after deastringency treatment. The response of POD enzyme was dependent on maturity stage, showing enhanced activity after CO2 treatment only for the fruit at the most mature stage. (C) 2014 Elsevier B.V. All rights reserved.
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spelling ReDivia42222025-04-25T14:42:35Z Deastringency treatment with CO2 induces oxidative stress in persimmon fruit Novillo, Pedro Salvador, Alejandra Magalhaes, Tahise Besada, Cristina Because of astringency at harvest, 'Rojo Brillante' persimmons are regularly submitted to deastringency treatment based on exposing fruit to a high CO2 concentration. The treatment conditions that ensure total astringency removal throughout the various maturity stages have been determined to be 95% CO2, 20 degrees C, 24 h. The aim of this study was to investigate changes in the redox state of persimmon fruit associated with this deastringency treatment. The levels of reactive oxygen species (ROS) (O-2(-) and H2O2), and the activities of the main ROS scavenging enzymes (CAT, POD, APX, and SOD), were determined at harvest and after deastringency in fruit at three different maturity stages. Our results showed that during 'Rojo Brillante' persimmon maturation, the level of O-2(-) gradually increased, while APX activity was lowered. The deastringency treatment with CO2 induced oxidative stress in the fruit, observed as an over-accumulation of O-2(-) and H2O2. As a response to ROS accumulation, the activities of the CAT, APX and SOD scavenging enzymes were up-regulated after deastringency treatment. The response of POD enzyme was dependent on maturity stage, showing enhanced activity after CO2 treatment only for the fruit at the most mature stage. (C) 2014 Elsevier B.V. All rights reserved. 2017-06-01T10:09:37Z 2017-06-01T10:09:37Z 2014 JUN 2014 article Novillo, P., Salvador, Alejandra, Magalhaes, Tahise, Besada, C. (2014). Deastringency treatment with CO2 induces oxidative stress in persimmon fruit. Postharvest Biology and Technology, 92, 16-22. 0925-5214 http://hdl.handle.net/20.500.11939/4222 10.1016/j.postharvbio.2013.12.019 en openAccess Impreso
spellingShingle Novillo, Pedro
Salvador, Alejandra
Magalhaes, Tahise
Besada, Cristina
Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title_full Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title_fullStr Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title_full_unstemmed Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title_short Deastringency treatment with CO2 induces oxidative stress in persimmon fruit
title_sort deastringency treatment with co2 induces oxidative stress in persimmon fruit
url http://hdl.handle.net/20.500.11939/4222
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