Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load
A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.11939/4186 |
| _version_ | 1855491709800022016 |
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| author | Munoz-Fambuena, Natalia Mesejo, Carlos Agustí, Manuel Tarraga, Susana Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. |
| author_browse | Agustí, Manuel González-Mas, María C. Iglesias, Domingo J. Mesejo, Carlos Munoz-Fambuena, Natalia Primo-Millo, Eduardo Tarraga, Susana |
| author_facet | Munoz-Fambuena, Natalia Mesejo, Carlos Agustí, Manuel Tarraga, Susana Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. |
| author_sort | Munoz-Fambuena, Natalia |
| collection | ReDivia |
| description | A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS–MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction. |
| format | Artículo |
| id | ReDivia4186 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | ReDivia41862025-04-25T14:42:29Z Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load Munoz-Fambuena, Natalia Mesejo, Carlos Agustí, Manuel Tarraga, Susana Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. A proteomic approach was used to know more about the molecular mechanism related to Citrus alternate bearing. To this end, we researched protein expression differences between on-crop and off-crop “Moncada” [Clementine ‘Oroval’ (Citrus clementina Hort ex Tanaka) x ‘Kara’ mandarin (Citrus unshiu Marc. x Citrus nobilis Lou.)] mandarin leaves. This variety usually shows a remarkable behaviour in alternate production. Samples were collected in the period during which the fruit affect flowering induction. From 2D DIGE gel, 110 spots were isolated: 43 showed increased expression in the off-crop samples compared to on-crop samples, while 67 showed increased expression in the on-crop samples against off-crop samples. These spots were identified by MALDI-MS or LC-MS–MS. According to the up-expressed proteins in off-crop leaves such as proteins related to nutrient reservoir activity or to the pentose phosphate pathway, the primary metabolism was more active in off-crop trees than in on-crop trees. In contrast, the proteins up-expressed in on-crop samples such as catalase were related to the oxidoreductase activity and, therefore, the redox state seemed different for off-crop and for on-crop leaves. Other proteins with unknown functions were isolated, which could be also related to the alternate bearing and to the flowering induction. 2017-06-01T10:09:32Z 2017-06-01T10:09:32Z 2013 JAN 2013 article Munoz-Fambuena, N., Mesejo, C., Agusti, M., Tarraga, S., Iglesias, D.J., Primo-Millo, E., Gonzalez-Mas, M.C. (2013). Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load. Plant Physiology and Biochemistry, 62, 95-106. 0981-9428 http://hdl.handle.net/20.500.11939/4186 10.1016/j.plaphy.2012.10.020 en openAccess Impreso |
| spellingShingle | Munoz-Fambuena, Natalia Mesejo, Carlos Agustí, Manuel Tarraga, Susana Iglesias, Domingo J. Primo-Millo, Eduardo González-Mas, María C. Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title | Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title_full | Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title_fullStr | Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title_full_unstemmed | Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title_short | Proteomic analysis of "Moncada" mandarin leaves with contrasting fruit load |
| title_sort | proteomic analysis of moncada mandarin leaves with contrasting fruit load |
| url | http://hdl.handle.net/20.500.11939/4186 |
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