TMV induces RNA decay pathways to modulate gene silencing and disease symptoms
RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC pathways. In plants, another important RNA degradation mechanism is the posttr...
| Main Authors: | , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/957 http://onlinelibrary.wiley.com/doi/10.1111/tpj.13323/epdf |
| _version_ | 1855034735736127488 |
|---|---|
| author | Conti, Gabriela Zavallo, Diego Venturuzzi, Andrea Laura Rodriguez, Maria Cecilia Crespi, Martin Asurmendi, Sebastian |
| author_browse | Asurmendi, Sebastian Conti, Gabriela Crespi, Martin Rodriguez, Maria Cecilia Venturuzzi, Andrea Laura Zavallo, Diego |
| author_facet | Conti, Gabriela Zavallo, Diego Venturuzzi, Andrea Laura Rodriguez, Maria Cecilia Crespi, Martin Asurmendi, Sebastian |
| author_sort | Conti, Gabriela |
| collection | INTA Digital |
| description | RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC pathways. In plants, another important RNA degradation mechanism is the posttranscriptional gene silencing (PTGS) mediated by small RNAs (siRNAs). Notably, the RQC pathway antagonizes PTGS by preventing the entry of dysfunctional mRNAs into the silencing pathway to avoid global degradation of mRNA by siRNAs. Viral transcripts must evade RNA degrading mechanisms, thus viruses encode PTGS suppressor proteins to counteract viral RNA silencing. Here, we demonstrate that tobacco plants infected with TMV and transgenic lines expressing TMV MP and CP (coat protein) proteins (which are not linked to the suppression of silencing) display increased transcriptional levels of RNA decay genes.
These plants also showed accumulation of cytoplasmic RNA granules with altered structure, increased rates of RNA decay for transgenes and defective transgene PTGS amplification. Furthermore, knockdown of RRP41 or RRP43 RNA exosome components led to lower levels of TMV accumulation with milder symptoms
after infection, several developmental defects and miRNA deregulation. Thus, we propose that TMV proteins induce RNA decay pathways (in particular exosome components) to impair antiviral PTGS and this defensive mechanism would constitute an additional counter-defense strategy that lead to disease
symptoms. |
| format | info:eu-repo/semantics/article |
| id | INTA957 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA9572017-12-20T14:43:39Z TMV induces RNA decay pathways to modulate gene silencing and disease symptoms Conti, Gabriela Zavallo, Diego Venturuzzi, Andrea Laura Rodriguez, Maria Cecilia Crespi, Martin Asurmendi, Sebastian Genética ARN Tobamovirus del Mosaico del Tabaco Virus de las Plantas Genetics RNA Tobacco Mosaic Tobamovirus Plant Viruses RNA decay pathways comprise a combination of RNA degradation mechanisms that are implicated in gene expression, development and defense responses in eukaryotes. These mechanisms are known as the RNA Quality Control or RQC pathways. In plants, another important RNA degradation mechanism is the posttranscriptional gene silencing (PTGS) mediated by small RNAs (siRNAs). Notably, the RQC pathway antagonizes PTGS by preventing the entry of dysfunctional mRNAs into the silencing pathway to avoid global degradation of mRNA by siRNAs. Viral transcripts must evade RNA degrading mechanisms, thus viruses encode PTGS suppressor proteins to counteract viral RNA silencing. Here, we demonstrate that tobacco plants infected with TMV and transgenic lines expressing TMV MP and CP (coat protein) proteins (which are not linked to the suppression of silencing) display increased transcriptional levels of RNA decay genes. These plants also showed accumulation of cytoplasmic RNA granules with altered structure, increased rates of RNA decay for transgenes and defective transgene PTGS amplification. Furthermore, knockdown of RRP41 or RRP43 RNA exosome components led to lower levels of TMV accumulation with milder symptoms after infection, several developmental defects and miRNA deregulation. Thus, we propose that TMV proteins induce RNA decay pathways (in particular exosome components) to impair antiviral PTGS and this defensive mechanism would constitute an additional counter-defense strategy that lead to disease symptoms. Inst. de Biotecnología Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Venturuzzi, Andrea Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Crespi, Martin. Institute of Plant Sciences Paris-Saclay; Francia Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-08-11T15:36:58Z 2017-08-11T15:36:58Z 2017 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/957 http://onlinelibrary.wiley.com/doi/10.1111/tpj.13323/epdf 1365-313X (Online) DOI: 10.1111/tpj.13323 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plant journal 89 (1) : 73–84. (2017) |
| spellingShingle | Genética ARN Tobamovirus del Mosaico del Tabaco Virus de las Plantas Genetics RNA Tobacco Mosaic Tobamovirus Plant Viruses Conti, Gabriela Zavallo, Diego Venturuzzi, Andrea Laura Rodriguez, Maria Cecilia Crespi, Martin Asurmendi, Sebastian TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title | TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title_full | TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title_fullStr | TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title_full_unstemmed | TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title_short | TMV induces RNA decay pathways to modulate gene silencing and disease symptoms |
| title_sort | tmv induces rna decay pathways to modulate gene silencing and disease symptoms |
| topic | Genética ARN Tobamovirus del Mosaico del Tabaco Virus de las Plantas Genetics RNA Tobacco Mosaic Tobamovirus Plant Viruses |
| url | http://hdl.handle.net/20.500.12123/957 http://onlinelibrary.wiley.com/doi/10.1111/tpj.13323/epdf |
| work_keys_str_mv | AT contigabriela tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms AT zavallodiego tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms AT venturuzziandrealaura tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms AT rodriguezmariacecilia tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms AT crespimartin tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms AT asurmendisebastian tmvinducesrnadecaypathwaystomodulategenesilencinganddiseasesymptoms |