Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina
Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the prese...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Springer
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/9461 https://link.springer.com/article/10.1007%2Fs00705-021-05002-4 https://doi.org/10.1007/s00705-021-05002-4 |
| _version_ | 1855484400409509888 |
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| author | Reyna, Pablo Gastón Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_browse | Bejerman, Nicolas Esteban Laguna, Irma Graciela Reyna, Pablo Gastón Rodriguez Pardina, Patricia |
| author_facet | Reyna, Pablo Gastón Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_sort | Reyna, Pablo Gastón |
| collection | INTA Digital |
| description | Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample. |
| format | Artículo |
| id | INTA9461 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA94612021-05-31T18:06:38Z Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina Reyna, Pablo Gastón Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Begomovirus Phaseolus vulgaris Virus del Mosaico Común Frijol Frijol (phaseolus) Bean Golden Mosaic Virus Beans Biological Control Argentina Control Biológico Región Noroeste, Argentina Common bean plants (Phaseolus vulgaris L.) showing different virus-like symptoms were collected in northwestern Argentina. Dot-blot hybridization tests showed that the begomoviruses bean golden mosaic virus and tomato yellow vein streak virus were the most prevalent, but they also revealed the presence of unknown begomoviruses. The complete genome sequence of one of these unknown begomoviruses was determined. Sequence analysis showed that the virus is a typical New World begomovirus, for which the name "bean bushy stunt virus" (BBSV) is proposed. Biological assays based on biolistic inoculations showed that BBSV induced leaf roll and stunting symptoms similar to those observed in the field-collected common bean sample. Instituto de Patología Vegetal Fil: Reyna, Pablo Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina. Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnica. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Fil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Rodriguez Pardina, Patricia.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA); Argentina. 2021-05-31T17:50:30Z 2021-05-31T17:50:30Z 2021-02-28 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9461 https://link.springer.com/article/10.1007%2Fs00705-021-05002-4 0304-8608 1432-8798 (online) https://doi.org/10.1007/s00705-021-05002-4 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Archives of Virology 166 : 1409–1414 (2021) |
| spellingShingle | Begomovirus Phaseolus vulgaris Virus del Mosaico Común Frijol Frijol (phaseolus) Bean Golden Mosaic Virus Beans Biological Control Argentina Control Biológico Región Noroeste, Argentina Reyna, Pablo Gastón Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title | Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title_full | Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title_fullStr | Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title_full_unstemmed | Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title_short | Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina |
| title_sort | biological and molecular characterization of bean bushy stunt virus a novel bipartite begomovirus infecting common bean in northwestern argentina |
| topic | Begomovirus Phaseolus vulgaris Virus del Mosaico Común Frijol Frijol (phaseolus) Bean Golden Mosaic Virus Beans Biological Control Argentina Control Biológico Región Noroeste, Argentina |
| url | http://hdl.handle.net/20.500.12123/9461 https://link.springer.com/article/10.1007%2Fs00705-021-05002-4 https://doi.org/10.1007/s00705-021-05002-4 |
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