Evaluation of a lyophilized CRISPR-Cas12 assay for a sensitive, specific, and rapid detection of SARS-CoV-2

We evaluated a lyophilized CRISPR-Cas12 assay for SARS-CoV-2 detection (Lyo-CRISPR SARS-CoV-2 kit) based on reverse transcription, isothermal amplification, and CRISPR-Cas12 reaction. From a total of 210 RNA samples extracted from nasopharyngeal swabs using spin columns, the Lyo-CRISPR SARS-CoV-2 ki...

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Bibliographic Details

Main Authors:	Curti, Lucía Ana, Primost, Ivana, Valla, Sofia, Ibañez Alegre, Daiana, Olguin Perglione, Cecilia, Repizo, Guillermo Daniel, Lara, Julia, Parcerisa, Ivana, Palacios, Antonela, Llases, María Eugenia, Rinflerch, Adriana, Barrios, Melanie, Pereyra Bonnet, Federico, Gimenez, Carla Alejandra, Marcone, Débora Natalia
Format:	Artículo
Language:	Inglés
Published:	MDPI 2021
Subjects:	CRISPR Severe Acute Respiratory Syndrome Coronavirus 2 COVID-19 Diagnosis Fluorescence Freeze Drying Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Interespaciadas Coronavirus del Síndrome Respiratorio Agudo Grave 2 Diagnóstico Fluorescencia Liofilización SARS-CoV-2
Online Access:	http://hdl.handle.net/20.500.12123/9128 https://www.mdpi.com/1999-4915/13/3/420 https://doi.org/10.3390/v13030420