Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene
The aim was to evaluate the effects of diet supplementation with red seaweed Pyropia columbina or β-carotene on the intestinal microbiota of juvenile Pacú (Piaractus mesopotamicus). Three hundred and fifteen fish (body weight 12.4 ± 2.8 g) were stocked in nine 300-L tanks with 35 fishes per tank and...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/9064 https://link.springer.com/article/10.1007/s10499-020-00508-1 https://doi.org/10.1007/s10499-020-00508-1 |
| _version_ | 1855036146347671552 |
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| author | Rossi, L.T. Romero Scharpen, Analía Zimmermann, Jorge Alberto Olivero, Carolina Raquel Zbrun, María Virginia Frizzo, Laureano Sebastian Signorini Porchiett, Marcelo Lisandro Bacchetta, Carla Cian, Raúl Esteban Cazenave, Jimena Soto, Lorena Paola Drago, Silvina |
| author_browse | Bacchetta, Carla Cazenave, Jimena Cian, Raúl Esteban Drago, Silvina Frizzo, Laureano Sebastian Olivero, Carolina Raquel Romero Scharpen, Analía Rossi, L.T. Signorini Porchiett, Marcelo Lisandro Soto, Lorena Paola Zbrun, María Virginia Zimmermann, Jorge Alberto |
| author_facet | Rossi, L.T. Romero Scharpen, Analía Zimmermann, Jorge Alberto Olivero, Carolina Raquel Zbrun, María Virginia Frizzo, Laureano Sebastian Signorini Porchiett, Marcelo Lisandro Bacchetta, Carla Cian, Raúl Esteban Cazenave, Jimena Soto, Lorena Paola Drago, Silvina |
| author_sort | Rossi, L.T. |
| collection | INTA Digital |
| description | The aim was to evaluate the effects of diet supplementation with red seaweed Pyropia columbina or β-carotene on the intestinal microbiota of juvenile Pacú (Piaractus mesopotamicus). Three hundred and fifteen fish (body weight 12.4 ± 2.8 g) were stocked in nine 300-L tanks with 35 fishes per tank and fed for 62 days with a basal feed (BG) or the same feed added with P. columbina (35 g kg−1) (AG) or supplemented with β-carotene (225 g kg−1) (β-CG). From fecal samples of three fishes slaughtered per group at 20th, 41th, and 62th days, plate counts of bacterial populations (enterobacteria, lactic acid bacteria—LAB, total aerobes, and psychrotrophs) and DGGE were made. Enterobacteria counts were lower in AG-group than the other groups throughout the experience (p = 0.016). LAB/enterobacteria ratio was greater in fishes supplemented with AG on 20th day, showing a greater amount of beneficial bacteria than non-beneficial ones. DGGE profiles resulting from the microbiota of β-CG at 62th day were clustered and separated from the rest of the profiles with a difference of 91%. Margalef richness (p = 0.049) and Shannon diversity index (p = 0.044) were lower in β-CG than in AG at 62th day. Taxonomic characterization showed the presence of three phylum: Bacteroidetes, Firmicutes, and Fusobacteria. This is the first study in which the microbiota of juvenile Pacú fed with biofunctional diets was evaluated. Future interventions that modify the bacterial ecosystem could improve the productive performance and ensure the safety of the pacu meat intended for consumption. |
| format | info:ar-repo/semantics/artículo |
| id | INTA9064 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA90642021-04-12T14:57:08Z Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene Rossi, L.T. Romero Scharpen, Analía Zimmermann, Jorge Alberto Olivero, Carolina Raquel Zbrun, María Virginia Frizzo, Laureano Sebastian Signorini Porchiett, Marcelo Lisandro Bacchetta, Carla Cian, Raúl Esteban Cazenave, Jimena Soto, Lorena Paola Drago, Silvina Piaractus mesopotamicus Alimentación de los Animales Carótenos Flora Intestinal Animal Feeding Red Seaweeds Carotenes Intestinal Flora Pacú Pyropia columbina Alga Roja Beta Carótenos Microbiota β-carotene The aim was to evaluate the effects of diet supplementation with red seaweed Pyropia columbina or β-carotene on the intestinal microbiota of juvenile Pacú (Piaractus mesopotamicus). Three hundred and fifteen fish (body weight 12.4 ± 2.8 g) were stocked in nine 300-L tanks with 35 fishes per tank and fed for 62 days with a basal feed (BG) or the same feed added with P. columbina (35 g kg−1) (AG) or supplemented with β-carotene (225 g kg−1) (β-CG). From fecal samples of three fishes slaughtered per group at 20th, 41th, and 62th days, plate counts of bacterial populations (enterobacteria, lactic acid bacteria—LAB, total aerobes, and psychrotrophs) and DGGE were made. Enterobacteria counts were lower in AG-group than the other groups throughout the experience (p = 0.016). LAB/enterobacteria ratio was greater in fishes supplemented with AG on 20th day, showing a greater amount of beneficial bacteria than non-beneficial ones. DGGE profiles resulting from the microbiota of β-CG at 62th day were clustered and separated from the rest of the profiles with a difference of 91%. Margalef richness (p = 0.049) and Shannon diversity index (p = 0.044) were lower in β-CG than in AG at 62th day. Taxonomic characterization showed the presence of three phylum: Bacteroidetes, Firmicutes, and Fusobacteria. This is the first study in which the microbiota of juvenile Pacú fed with biofunctional diets was evaluated. Future interventions that modify the bacterial ecosystem could improve the productive performance and ensure the safety of the pacu meat intended for consumption. EEA Rafaela Fil: Rossi, L.T. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Rossi, L.T. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Romero Scharpen, Analía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Romero Scharpen, Analía. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Zimmermann, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Zimmermann, Jorge Alberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Olivero, Carolina Raquel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Olivero, Carolina Raquel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Zbrun, María Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral: Laboratorio de Análisis de Alimentos; Argentina Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Frizzo, Laureano Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos, Argentina Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Frizzo, Laureano Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Signorini, Marcelo. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Signorini, Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Bacchetta, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Nacional de Limnología; Argentina Fil: Bacchetta, Carla. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Cian, Raúl Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Tecnología de Alimentos; Argentina Fil: Cian, Raúl Esteban. Universidad Nacional del Litoral. Instituto de Tecnología de Alimentosl; Argentina Fil: Cazenave, Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto Nacional de Limnología; Argentina Fil: Cazenave, Jimena. Universidad Nacional del Litoral. Instituto Nacional de Limnología; Argentina Fil: Soto, Lorena Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Análisis de Alimentos ; Argentina Fil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública; Argentina Fil: Drago, Silvina. Universidad Nacional del Litoral. Facultad de Ciencias Químicas. Instituto de Tecnología de los Alimentos; Argentina Fil: Drago, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Tecnología de Alimentos; Argentina 2021-04-12T14:49:33Z 2021-04-12T14:49:33Z 2020-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/9064 https://link.springer.com/article/10.1007/s10499-020-00508-1 0967-6120 1573-143X https://doi.org/10.1007/s10499-020-00508-1 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Aquaculture International 28 : 1001–1016. (2020) |
| spellingShingle | Piaractus mesopotamicus Alimentación de los Animales Carótenos Flora Intestinal Animal Feeding Red Seaweeds Carotenes Intestinal Flora Pacú Pyropia columbina Alga Roja Beta Carótenos Microbiota β-carotene Rossi, L.T. Romero Scharpen, Analía Zimmermann, Jorge Alberto Olivero, Carolina Raquel Zbrun, María Virginia Frizzo, Laureano Sebastian Signorini Porchiett, Marcelo Lisandro Bacchetta, Carla Cian, Raúl Esteban Cazenave, Jimena Soto, Lorena Paola Drago, Silvina Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title | Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title_full | Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title_fullStr | Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title_full_unstemmed | Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title_short | Intestinal microbiota modulation in juvenile Pacú (Piaractus mesopotamicus) by supplementation with Pyropia columbina and β-carotene |
| title_sort | intestinal microbiota modulation in juvenile pacu piaractus mesopotamicus by supplementation with pyropia columbina and β carotene |
| topic | Piaractus mesopotamicus Alimentación de los Animales Carótenos Flora Intestinal Animal Feeding Red Seaweeds Carotenes Intestinal Flora Pacú Pyropia columbina Alga Roja Beta Carótenos Microbiota β-carotene |
| url | http://hdl.handle.net/20.500.12123/9064 https://link.springer.com/article/10.1007/s10499-020-00508-1 https://doi.org/10.1007/s10499-020-00508-1 |
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