Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems

Langmuir and Langmuir-Blodgett films holding a synthetic bioinspired wound healing active compound were used as drug-delivery platforms. Palmitic acid Langmuir monolayers were able to incorporate 2-methyltriclisine, a synthetic Triclisine derivative that showed wound healing activity. The layers pro...

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Main Authors: Fernández, Luciana, Reviglio, Ana Lucía, Heredia, Daniel A., Morales, Gustavo M., Santo, Marisa, Otero, Luis, Alustiza, Fabrisio Eduardo, Liaudat, Ana Cecilia, Bosch, Pablo, Larghi, Enrique L., Bracca, Andrea B.J., Kaufman, Teodoro S.
Format: Artículo
Language:Inglés
Published: Elsevier 2021
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/8902
https://www.sciencedirect.com/science/article/pii/S2405844021005417
https://doi.org/10.1016/j.heliyon.2021.e06436
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author Fernández, Luciana
Reviglio, Ana Lucía
Heredia, Daniel A.
Morales, Gustavo M.
Santo, Marisa
Otero, Luis
Alustiza, Fabrisio Eduardo
Liaudat, Ana Cecilia
Bosch, Pablo
Larghi, Enrique L.
Bracca, Andrea B.J.
Kaufman, Teodoro S.
author_browse Alustiza, Fabrisio Eduardo
Bosch, Pablo
Bracca, Andrea B.J.
Fernández, Luciana
Heredia, Daniel A.
Kaufman, Teodoro S.
Larghi, Enrique L.
Liaudat, Ana Cecilia
Morales, Gustavo M.
Otero, Luis
Reviglio, Ana Lucía
Santo, Marisa
author_facet Fernández, Luciana
Reviglio, Ana Lucía
Heredia, Daniel A.
Morales, Gustavo M.
Santo, Marisa
Otero, Luis
Alustiza, Fabrisio Eduardo
Liaudat, Ana Cecilia
Bosch, Pablo
Larghi, Enrique L.
Bracca, Andrea B.J.
Kaufman, Teodoro S.
author_sort Fernández, Luciana
collection INTA Digital
description Langmuir and Langmuir-Blodgett films holding a synthetic bioinspired wound healing active compound were used as drug-delivery platforms. Palmitic acid Langmuir monolayers were able to incorporate 2-methyltriclisine, a synthetic Triclisine derivative that showed wound healing activity. The layers proved to be stable and the nanocomposites were transferred to solid substrates. Normal human lung cells (Medical Research Council cell strain 5, MRC-5) were grown over the monomolecular Langmuir-Blodgett films that acted as a drug reservoir and delivery system. The proliferation and migration of the cells were clearly affected by the presence of 2-methyltriclisine in the amphiphilic layers. The methodology is proposed as a simple and reliable model for the study of the effects of bioactive compounds over cellular cultures.
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institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
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spelling INTA89022021-03-16T14:33:54Z Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems Fernández, Luciana Reviglio, Ana Lucía Heredia, Daniel A. Morales, Gustavo M. Santo, Marisa Otero, Luis Alustiza, Fabrisio Eduardo Liaudat, Ana Cecilia Bosch, Pablo Larghi, Enrique L. Bracca, Andrea B.J. Kaufman, Teodoro S. Cultivo de Células Compuestos Bioactivos Sistemas de Administración de Drogas Transportadores de Fármacos Cell Culture Bioactive Compounds Drug Delivery Systems Drug Carriers Langmuir and Langmuir-Blodgett films holding a synthetic bioinspired wound healing active compound were used as drug-delivery platforms. Palmitic acid Langmuir monolayers were able to incorporate 2-methyltriclisine, a synthetic Triclisine derivative that showed wound healing activity. The layers proved to be stable and the nanocomposites were transferred to solid substrates. Normal human lung cells (Medical Research Council cell strain 5, MRC-5) were grown over the monomolecular Langmuir-Blodgett films that acted as a drug reservoir and delivery system. The proliferation and migration of the cells were clearly affected by the presence of 2-methyltriclisine in the amphiphilic layers. The methodology is proposed as a simple and reliable model for the study of the effects of bioactive compounds over cellular cultures. EEA Marcos Juárez Fil: Fernández, Luciana. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina Fil: Fernández, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Reviglio, Ana Lucía. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina Fil: Reviglio, Ana Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Heredia, Daniel A. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina. Fil: Heredia, Daniel A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Morales, Gustavo M. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina Fil: Morales, Gustavo M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Santo, Marisa. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina Fil: Santo, Marisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Otero, Luis. Universidad Nacional de Río Cuarto. Departamento de Física y Departamento de Química; Argentina Fil: Otero, Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina Fil: Larghi, Enrique L. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Bracca, Andrea B.J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina Fil: Kaufman, Teodoro S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Química Rosario; Argentina 2021-03-16T14:29:10Z 2021-03-16T14:29:10Z 2021-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8902 https://www.sciencedirect.com/science/article/pii/S2405844021005417 2405-8440 https://doi.org/10.1016/j.heliyon.2021.e06436 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Heliyon 7 (3) : e06436 (March 2021)
spellingShingle Cultivo de Células
Compuestos Bioactivos
Sistemas de Administración de Drogas
Transportadores de Fármacos
Cell Culture
Bioactive Compounds
Drug Delivery Systems
Drug Carriers
Fernández, Luciana
Reviglio, Ana Lucía
Heredia, Daniel A.
Morales, Gustavo M.
Santo, Marisa
Otero, Luis
Alustiza, Fabrisio Eduardo
Liaudat, Ana Cecilia
Bosch, Pablo
Larghi, Enrique L.
Bracca, Andrea B.J.
Kaufman, Teodoro S.
Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title_full Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title_fullStr Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title_full_unstemmed Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title_short Langmuir-Blodgett monolayers holding a wound healing active compound and its effect in cell culture. A model for the study of surface mediated drug delivery systems
title_sort langmuir blodgett monolayers holding a wound healing active compound and its effect in cell culture a model for the study of surface mediated drug delivery systems
topic Cultivo de Células
Compuestos Bioactivos
Sistemas de Administración de Drogas
Transportadores de Fármacos
Cell Culture
Bioactive Compounds
Drug Delivery Systems
Drug Carriers
url http://hdl.handle.net/20.500.12123/8902
https://www.sciencedirect.com/science/article/pii/S2405844021005417
https://doi.org/10.1016/j.heliyon.2021.e06436
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