Cover Image
QR Code

Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1

Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Ps...

Full description

Main Authors: Ayub, Nicolás Daniel, Fox, Ana Romina, Garcia, Araceli Nora, Mozzicafreddo, Matteo, Cuccioloni, Massimiliano, Angeletti, Mauro, Pagano, Elba Maria, Soto, Gabriela Cynthia
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Oxford University Press 2021
Subjects:
Immunity
Antimicrobial Agents
Peptides
Defence Mechanisms
Inmunidad
Agente Antimicrobiano
Péptidos
Pseudomonas
Mecanismos de Defensa
Medicago sativa
Alfalfa
Online Access:http://hdl.handle.net/20.500.12123/8712
https://academic.oup.com/femsle/article/362/2/1/2467374
https://doi.org/10.1093/femsle/fnu006
Summary:Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties.

Similar Items