Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1
Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Ps...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8712 https://academic.oup.com/femsle/article/362/2/1/2467374 https://doi.org/10.1093/femsle/fnu006 |
| _version_ | 1855036085020655616 |
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| author | Ayub, Nicolás Daniel Fox, Ana Romina Garcia, Araceli Nora Mozzicafreddo, Matteo Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Soto, Gabriela Cynthia |
| author_browse | Angeletti, Mauro Ayub, Nicolás Daniel Cuccioloni, Massimiliano Fox, Ana Romina Garcia, Araceli Nora Mozzicafreddo, Matteo Pagano, Elba Maria Soto, Gabriela Cynthia |
| author_facet | Ayub, Nicolás Daniel Fox, Ana Romina Garcia, Araceli Nora Mozzicafreddo, Matteo Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Soto, Gabriela Cynthia |
| author_sort | Ayub, Nicolás Daniel |
| collection | INTA Digital |
| description | Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8712 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Oxford University Press |
| publisherStr | Oxford University Press |
| record_format | dspace |
| spelling | INTA87122021-02-22T12:53:33Z Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 Ayub, Nicolás Daniel Fox, Ana Romina Garcia, Araceli Nora Mozzicafreddo, Matteo Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Soto, Gabriela Cynthia Immunity Antimicrobial Agents Peptides Defence Mechanisms Inmunidad Agente Antimicrobiano Péptidos Pseudomonas Mecanismos de Defensa Medicago sativa Alfalfa Snakin-1, a peptide produced by higher plants, has broad-spectrum antibiotic activity, inhibiting organisms ranging from Bacteria to Eukaryotes. However, the mode of action against target organisms is poorly understood. As a first step to elucidate the mechanism, we screened a mutation library of Pseudomonas fluorescens Pf-5 in LB and agar medium supplemented with alfalfa snakin-1 (MsSN1). We identified three biofilm formation-related Pseudomonas mutants that showed increased resistance to MsSN1. Genetic, physiological and bioinformatics analysis validated the results of the mutant screens, indicating that bacterial adhesion protein lapA is probably the target of MsSN1. Collectively, these findings suggest that snakin-1 acts on microbial adhesion properties. Instituto de Genética Fil: Ayub, Nicolás Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fox, Ana Romina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Araceli Nora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Mozzicafreddo, Matteo. University of Camerino. School of Biosciences and Biotechnology; Italia Fil: Cuccioloni, Massimiliano. University of Camerino. School of Biosciences and Biotechnology; Italia Fil: Angeletti, Mauro. University of Camerino. School of Biosciences and Biotechnology; Italia Fil: Pagano, Elba Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Soto, Gabriela Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-02-22T12:43:22Z 2021-02-22T12:43:22Z 2015-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8712 https://academic.oup.com/femsle/article/362/2/1/2467374 1574-6968 https://doi.org/10.1093/femsle/fnu006 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford University Press FEMS Microbiology Letters 362 (2) : 1-6 (Enero 2015) |
| spellingShingle | Immunity Antimicrobial Agents Peptides Defence Mechanisms Inmunidad Agente Antimicrobiano Péptidos Pseudomonas Mecanismos de Defensa Medicago sativa Alfalfa Ayub, Nicolás Daniel Fox, Ana Romina Garcia, Araceli Nora Mozzicafreddo, Matteo Cuccioloni, Massimiliano Angeletti, Mauro Pagano, Elba Maria Soto, Gabriela Cynthia Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title | Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title_full | Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title_fullStr | Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title_full_unstemmed | Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title_short | Pseudomonas fluorescens Pf-5 genome-wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin-1 |
| title_sort | pseudomonas fluorescens pf 5 genome wide mutant screen for resistance to the antimicrobial peptide alfalfa snakin 1 |
| topic | Immunity Antimicrobial Agents Peptides Defence Mechanisms Inmunidad Agente Antimicrobiano Péptidos Pseudomonas Mecanismos de Defensa Medicago sativa Alfalfa |
| url | http://hdl.handle.net/20.500.12123/8712 https://academic.oup.com/femsle/article/362/2/1/2467374 https://doi.org/10.1093/femsle/fnu006 |
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