Virus infection elevates transcriptional activity of miR164a promoter in plants
Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-tran...
| Autores principales: | , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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BioMed Central
2021
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| Acceso en línea: | https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-152 http://hdl.handle.net/20.500.12123/8665 https://doi.org/10.1186/1471-2229-9-152 |
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| author | Bazzini, Ariel Alejandro Almasia, Natalia Ines Manacorda, Carlos Augusto Mongelli, Vanesa Claudia Conti, Gabriela Maroniche, Guillermo Andrés Rodriguez, Maria Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastian |
| author_browse | Almasia, Natalia Ines Asurmendi, Sebastian Bazzini, Ariel Alejandro Conti, Gabriela Del Vas, Mariana Distefano, Ana Julia Hopp, Horacio Esteban Manacorda, Carlos Augusto Maroniche, Guillermo Andrés Mongelli, Vanesa Claudia Rodriguez, Maria Cecilia |
| author_facet | Bazzini, Ariel Alejandro Almasia, Natalia Ines Manacorda, Carlos Augusto Mongelli, Vanesa Claudia Conti, Gabriela Maroniche, Guillermo Andrés Rodriguez, Maria Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastian |
| author_sort | Bazzini, Ariel Alejandro |
| collection | INTA Digital |
| description | Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations.
Results: Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction.
Conclusion: This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. |
| format | Artículo |
| id | INTA8665 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | BioMed Central |
| publisherStr | BioMed Central |
| record_format | dspace |
| spelling | INTA86652021-02-17T13:40:14Z Virus infection elevates transcriptional activity of miR164a promoter in plants Bazzini, Ariel Alejandro Almasia, Natalia Ines Manacorda, Carlos Augusto Mongelli, Vanesa Claudia Conti, Gabriela Maroniche, Guillermo Andrés Rodriguez, Maria Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastian Transgenic Plants Tobacco Mosaic Tobamovirus Transcription Plantas Transgénicas Tobamovirus del Mosaico del Tabaco Transcripción Background: Micro RNAs (miRs) constitute a large group of endogenous small RNAs that have crucial roles in many important plant functions. Virus infection and transgenic expression of viral proteins alter accumulation and activity of miRs and so far, most of the published evidence involves post-transcriptional regulations. Results: Using transgenic plants expressing a reporter gene under the promoter region of a characterized miR (P-miR164a), we monitored the reporter gene expression in different tissues and during Arabidopsis development. Strong expression was detected in both vascular tissues and hydathodes. P-miR164a activity was developmentally regulated in plants with a maximum expression at stages 1.12 to 5.1 (according to Boyes, 2001) along the transition from vegetative to reproductive growth. Upon quantification of P-miR164a-derived GUS activity after Tobacco mosaic virus Cg or Oilseed rape mosaic virus (ORMV) infection and after hormone treatments, we demonstrated that ORMV and gibberellic acid elevated P-miR164a activity. Accordingly, total mature miR164, precursor of miR164a and CUC1 mRNA (a miR164 target) levels increased after virus infection and interestingly the most severe virus (ORMV) produced the strongest promoter induction. Conclusion: This work shows for the first time that the alteration of miR pathways produced by viral infections possesses a transcriptional component. In addition, the degree of miR alteration correlates with virus severity since a more severe virus produces a stronger P-miR164a induction. Instituto de Biotecnología Fil: Bazzini, Ariel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Almasia, Natalia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Mongelli, Vanesa Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Maroniche, Guillermo Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2021-02-17T13:35:30Z 2021-02-17T13:35:30Z 2009-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-152 http://hdl.handle.net/20.500.12123/8665 1471-2229 https://doi.org/10.1186/1471-2229-9-152 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioMed Central BMC Plant Biology 9 : 152 (Diciembre 2009) |
| spellingShingle | Transgenic Plants Tobacco Mosaic Tobamovirus Transcription Plantas Transgénicas Tobamovirus del Mosaico del Tabaco Transcripción Bazzini, Ariel Alejandro Almasia, Natalia Ines Manacorda, Carlos Augusto Mongelli, Vanesa Claudia Conti, Gabriela Maroniche, Guillermo Andrés Rodriguez, Maria Cecilia Distefano, Ana Julia Hopp, Horacio Esteban Del Vas, Mariana Asurmendi, Sebastian Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title | Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title_full | Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title_fullStr | Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title_full_unstemmed | Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title_short | Virus infection elevates transcriptional activity of miR164a promoter in plants |
| title_sort | virus infection elevates transcriptional activity of mir164a promoter in plants |
| topic | Transgenic Plants Tobacco Mosaic Tobamovirus Transcription Plantas Transgénicas Tobamovirus del Mosaico del Tabaco Transcripción |
| url | https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-9-152 http://hdl.handle.net/20.500.12123/8665 https://doi.org/10.1186/1471-2229-9-152 |
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