A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis
LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technolog...
| Autores principales: | , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/8141 https://link.springer.com/protocol/10.1007%2F978-1-4939-7037-7_14 https://doi.org/10.1007/978-1-4939-7037-7_14 |
| _version_ | 1855035982886207488 |
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| author | Trangoni, Marcos David Gioffre, Andrea Cravero, Silvio Lorenzo Pedro |
| author_browse | Cravero, Silvio Lorenzo Pedro Gioffre, Andrea Trangoni, Marcos David |
| author_facet | Trangoni, Marcos David Gioffre, Andrea Cravero, Silvio Lorenzo Pedro |
| author_sort | Trangoni, Marcos David |
| collection | INTA Digital |
| description | LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8141 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA81412020-10-28T21:23:17Z A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis Trangoni, Marcos David Gioffre, Andrea Cravero, Silvio Lorenzo Pedro Isothermal Processes Molecular Biology Brucellosis Procesos Isotermales Biología Molecular Brucella Mycobacterium avium subsp. paratuberculosis Brucelosis Paratuberculosis Isothermal Amplification Amplificación Isotermal LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine. Instituto de Biotecnología Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina 2020-10-28T21:12:49Z 2020-10-28T21:12:49Z 2017-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8141 https://link.springer.com/protocol/10.1007%2F978-1-4939-7037-7_14 1064-3745 https://doi.org/10.1007/978-1-4939-7037-7_14 eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Methods in Molecular Biology 1616 : 221-229 (Junio 2017) |
| spellingShingle | Isothermal Processes Molecular Biology Brucellosis Procesos Isotermales Biología Molecular Brucella Mycobacterium avium subsp. paratuberculosis Brucelosis Paratuberculosis Isothermal Amplification Amplificación Isotermal Trangoni, Marcos David Gioffre, Andrea Cravero, Silvio Lorenzo Pedro A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title | A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title_full | A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title_fullStr | A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title_full_unstemmed | A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title_short | A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis |
| title_sort | closed tube loop mediated isothermal amplification assay for the visual endpoint detection of brucella spp and mycobacterium avium subsp paratuberculosis |
| topic | Isothermal Processes Molecular Biology Brucellosis Procesos Isotermales Biología Molecular Brucella Mycobacterium avium subsp. paratuberculosis Brucelosis Paratuberculosis Isothermal Amplification Amplificación Isotermal |
| url | http://hdl.handle.net/20.500.12123/8141 https://link.springer.com/protocol/10.1007%2F978-1-4939-7037-7_14 https://doi.org/10.1007/978-1-4939-7037-7_14 |
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