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A closed-tube loop-mediated isothermal amplification assay for the visual endpoint detection of Brucella spp. and Mycobacterium avium subsp. paratuberculosis

LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technolog...

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Main Authors: Trangoni, Marcos David, Gioffre, Andrea, Cravero, Silvio Lorenzo Pedro
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Springer 2020
Subjects:
Isothermal Processes
Molecular Biology
Brucellosis
Procesos Isotermales
Biología Molecular
Brucella
Mycobacterium avium subsp. paratuberculosis
Brucelosis
Paratuberculosis
Isothermal Amplification
Amplificación Isotermal
Online Access:http://hdl.handle.net/20.500.12123/8141
https://link.springer.com/protocol/10.1007%2F978-1-4939-7037-7_14
https://doi.org/10.1007/978-1-4939-7037-7_14
Summary:LAMP (loop-mediated isothermal amplification) is an isothermal nucleic acid amplification technique that is characterized by its efficiency, rapidity, high yield of final product, robustness, sensitivity, and specificity, with the blueprint that it can be implemented in laboratories of low technological complexity. Despite the conceptual complexity underlying the mechanistic basis for the nucleic acid amplification, the technique is simple to use and the amplification and detection can be carried out in just one step. In this chapter, we present a protocol based on LAMP for the rapid identification of isolates of Brucella spp. and Mycobacterium avium subsp. paratuberculosis, two major bacterial pathogens in veterinary medicine.

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