First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina
The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Alemán |
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American Phytopathological Society
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/8103 https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN https://doi.org/10.1094/PDIS-06-19-1207-PDN |
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| author | Bernanrdi Lima, Nelson Pastor, Silvina Estela Maza, Claudia Elizabeth Conforto, Erica Cinthia Vargas Gil, Silvina Roca, Monica |
| author_browse | Bernanrdi Lima, Nelson Conforto, Erica Cinthia Maza, Claudia Elizabeth Pastor, Silvina Estela Roca, Monica Vargas Gil, Silvina |
| author_facet | Bernanrdi Lima, Nelson Pastor, Silvina Estela Maza, Claudia Elizabeth Conforto, Erica Cinthia Vargas Gil, Silvina Roca, Monica |
| author_sort | Bernanrdi Lima, Nelson |
| collection | INTA Digital |
| description | The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced. |
| format | info:ar-repo/semantics/artículo |
| id | INTA8103 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Alemán |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | American Phytopathological Society |
| publisherStr | American Phytopathological Society |
| record_format | dspace |
| spelling | INTA81032020-10-22T11:36:29Z First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina Bernanrdi Lima, Nelson Pastor, Silvina Estela Maza, Claudia Elizabeth Conforto, Erica Cinthia Vargas Gil, Silvina Roca, Monica Colletotrichum Olea Europaea Phylogeny Pathogenicity Filogenia Patogenicidad Argentina Colletotrichum Gloeosporioides Species Complex Phylogenetic Analysis Olivo The olive (Olea europaea L.), family Oleaceae, is an important crop in Argentina, mainly in the production of olive oils and table olives. In the country, that economic loss to the olive industry caused by anthracnose is estimated to be over $9 million dollars a year. During the harvest of 2018 to 2019, severe symptoms of anthracnose were observed with an incidence of 73% on 483 olive tree (cv. Manzanilla) in a commercial orchard located in Capital, La Rioja, Argentina. Lesions on olive fruits were irregular, becoming dark brown and depressed, with mature fruit mummification, being typical lesions of anthracnose. For fungal isolation, conidia were collected from orange masses of spores, in acervuli, from 20 infected fruits of 10 olive tree, and were placed in Petri plates containing potato dextrose agar (PDA). Plates were incubated at 25°C in the dark for 6 days, and colonies that were morphologically similar to species of Colletotrichum were transferred to PDA. Three isolates were obtained and then single-spore purified. The isolates (IPAVE 071, IPAVE 072, and IPAVE 076) were preserved and deposited in the Culture Collection of Instituto de Patología Vegetal (IPAVE) at the Instituto Nacional de Tecnología Agropecuaria (INTA) (Córdoba, Argentina). Colonies presented mycelium that were flat with a white margin, and gray aerial mycelium. Conidia were hyaline, aseptate, straight, subcylindrical and clavate, (12.3 to) 13.9 to 19.1 (to 20.57) × (3.5 to) 4.1 to 5.61 (to 6.1) µm, mean ± SD = 14.8 ± 0.2 × 4.8 ± 0.1 µm, length/width ratio = 3.1 (n = 50). Morphological characterization was consistent with the description of Colletotrichum theobromicola (Rojas et al. 2010). For molecular identification, gene sequences were obtained from the partial glyceraldehyde-3-phosphate dehydrogenase (GAPDH), actin (ACT), and β-tubulin 2 (TUB2) regions, which were amplified by PCR (Weir et al. 2012) and sequenced. Instituto de Patología Vegetal Fil: Bernanrdi Lima, Nelson. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Fil: Maza, Claudia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Chilecito; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFYMA). Córdoba; Argentina Fil: Roca, Monica. Universidad Nacional La Rioja. Servicio Nacional de Sanidad y Calidad Agroalimentaria; Argentina 2020-10-22T11:26:36Z 2020-10-22T11:26:36Z 2020-01-31 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/8103 https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN 0191-2917 1943-7692 https://doi.org/10.1094/PDIS-06-19-1207-PDN deu info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Phytopathological Society Plant Disease 104 (2) : (February 2020 ) |
| spellingShingle | Colletotrichum Olea Europaea Phylogeny Pathogenicity Filogenia Patogenicidad Argentina Colletotrichum Gloeosporioides Species Complex Phylogenetic Analysis Olivo Bernanrdi Lima, Nelson Pastor, Silvina Estela Maza, Claudia Elizabeth Conforto, Erica Cinthia Vargas Gil, Silvina Roca, Monica First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title | First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title_full | First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title_fullStr | First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title_full_unstemmed | First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title_short | First Report of Anthracnose of Olive Fruit Caused by Colletotrichum theobromicola in Argentina |
| title_sort | first report of anthracnose of olive fruit caused by colletotrichum theobromicola in argentina |
| topic | Colletotrichum Olea Europaea Phylogeny Pathogenicity Filogenia Patogenicidad Argentina Colletotrichum Gloeosporioides Species Complex Phylogenetic Analysis Olivo |
| url | http://hdl.handle.net/20.500.12123/8103 https://apsjournals.apsnet.org/doi/10.1094/PDIS-06-19-1207-PDN https://doi.org/10.1094/PDIS-06-19-1207-PDN |
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