A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness
Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Hindawi
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7980 https://www.hindawi.com/journals/bmri/2020/4741237/ https://doi.org/10.1155/2020/4741237 |
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| author | Bei, Jinlong Bigi, María Mercedes Lima, Analía Zhang, Qi Blanco, Federico Carlos López, Beatriz Yu, Ting Wang, Zhilin Dai, Zhangyan Chen, Zhuang Cataldi, Angel Adrian Sasiain, María del Carmen Ritacco, Viviana De la Barrera, Silvia Susana Soria, Marcelo Abel Durán, Rosario Bigi, Fabiana |
| author_browse | Bei, Jinlong Bigi, Fabiana Bigi, María Mercedes Blanco, Federico Carlos Cataldi, Angel Adrian Chen, Zhuang Dai, Zhangyan De la Barrera, Silvia Susana Durán, Rosario Lima, Analía López, Beatriz Ritacco, Viviana Sasiain, María del Carmen Soria, Marcelo Abel Wang, Zhilin Yu, Ting Zhang, Qi |
| author_facet | Bei, Jinlong Bigi, María Mercedes Lima, Analía Zhang, Qi Blanco, Federico Carlos López, Beatriz Yu, Ting Wang, Zhilin Dai, Zhangyan Chen, Zhuang Cataldi, Angel Adrian Sasiain, María del Carmen Ritacco, Viviana De la Barrera, Silvia Susana Soria, Marcelo Abel Durán, Rosario Bigi, Fabiana |
| author_sort | Bei, Jinlong |
| collection | INTA Digital |
| description | Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses. |
| format | Artículo |
| id | INTA7980 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Hindawi |
| publisherStr | Hindawi |
| record_format | dspace |
| spelling | INTA79802020-09-28T16:45:21Z A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness Bei, Jinlong Bigi, María Mercedes Lima, Analía Zhang, Qi Blanco, Federico Carlos López, Beatriz Yu, Ting Wang, Zhilin Dai, Zhangyan Chen, Zhuang Cataldi, Angel Adrian Sasiain, María del Carmen Ritacco, Viviana De la Barrera, Silvia Susana Soria, Marcelo Abel Durán, Rosario Bigi, Fabiana Genetic Isolation Drug Resistance Biological Properties Aislamiento Genético Resistencia a Medicamentos Mycobacterium tuberculosis Propiedades Biológicas Molecular Properties Propiedades Moleculares Tuberculosis (TB) is an infectious disease, caused by Mycobacterium tuberculosis, primarily affecting the lungs. The M. tuberculosis strain of the Haarlem family named M was responsible for a large multidrug-resistant TB (MDR-TB) outbreak in Buenos Aires. This outbreak started in the early 1990s and in the mid 2000s still accounted for 29% of all MDR-TB cases in Argentina. By contrast, a clonal variant of strain M, named 410, has caused a single tuberculosis case since the onset of the outbreak. The molecular bases of the high epidemiological fitness of the M strain remain unclear. To assess its unique molecular properties, herein, we performed a comparative protein and lipid analysis of a representative clone of the M strain (Mp) and the nonprosperous M variant 410. We also evaluated their growth in low pH. The variant 410 had higher levels of latency proteins under standard conditions and delayed growth at low pH, suggesting that it is more sensitive to stress stimuli than Mp. Moreover, Mp showed higher levels of mycolic acids covalently attached to the cell wall and lower accumulation of free mycolic acids in the outer layer than the 410 strain. The low expression of latency proteins together with the reduced content of surface mycolic acids may facilitate Mp to evade the host immune responses. Instituto de Biotecnología Fil: Bei, Jinlong. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina Fil: Lima, Analía. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay Fil: Zhang, Qi. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: López, Beatriz. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina Fil: Yu, Ting. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Wang, Zhilin. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Dai, Zhangyan. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Chen, Zhuang. Guangdong Academy of Agricultural Sciences (GDAAS). AGRO-Biological Gene Research Center; China Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sasiain, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Ritacco, Viviana. Administración Nacional de Laboratorios e Institutos de Salud. Instituto Nacional de Enfermedades Infecciosas “Dr. Carlos G. Malbrán”; Argentina Fil: de la Barrera, Silvia Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos; Argentina Fil: Durán, Rosario. Institut Pasteur de Montevideo; Uruguay. Instituto de Investigaciones Biológicas Clemente Estable; Uruguay Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-09-28T16:37:46Z 2020-09-28T16:37:46Z 2020-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7980 https://www.hindawi.com/journals/bmri/2020/4741237/ 2314-6141 https://doi.org/10.1155/2020/4741237 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Hindawi Biomed Research International 2020 : 4741237 (Abril 2020) |
| spellingShingle | Genetic Isolation Drug Resistance Biological Properties Aislamiento Genético Resistencia a Medicamentos Mycobacterium tuberculosis Propiedades Biológicas Molecular Properties Propiedades Moleculares Bei, Jinlong Bigi, María Mercedes Lima, Analía Zhang, Qi Blanco, Federico Carlos López, Beatriz Yu, Ting Wang, Zhilin Dai, Zhangyan Chen, Zhuang Cataldi, Angel Adrian Sasiain, María del Carmen Ritacco, Viviana De la Barrera, Silvia Susana Soria, Marcelo Abel Durán, Rosario Bigi, Fabiana A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title | A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title_full | A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title_fullStr | A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title_full_unstemmed | A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title_short | A phenotypic characterization of two isolates of a multidrug-resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| title_sort | phenotypic characterization of two isolates of a multidrug resistant outbreak strain of mycobacterium tuberculosis with opposite epidemiological fitness |
| topic | Genetic Isolation Drug Resistance Biological Properties Aislamiento Genético Resistencia a Medicamentos Mycobacterium tuberculosis Propiedades Biológicas Molecular Properties Propiedades Moleculares |
| url | http://hdl.handle.net/20.500.12123/7980 https://www.hindawi.com/journals/bmri/2020/4741237/ https://doi.org/10.1155/2020/4741237 |
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