High genetic diversity and differentiation of the babesia ovis population in Turkey
Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Wiley
2020
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| Online Access: | http://hdl.handle.net/20.500.12123/7778 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13174 https://doi.org/10.1111/tbed.13174 |
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| author | Mira, Anabela Unlu, Ahmet Hakan Bilgic, Huseyin Bilgin Bakirci, Serkan Hacilarlioglu, Selin Karagenc, Tulin Carletti, Tamara Weir, William Shiels, Brian Shkap, Varda Aktas, Munir Florin‐Christensen, Mónica Schnittger, Leonhard |
| author_browse | Aktas, Munir Bakirci, Serkan Bilgic, Huseyin Bilgin Carletti, Tamara Florin‐Christensen, Mónica Hacilarlioglu, Selin Karagenc, Tulin Mira, Anabela Schnittger, Leonhard Shiels, Brian Shkap, Varda Unlu, Ahmet Hakan Weir, William |
| author_facet | Mira, Anabela Unlu, Ahmet Hakan Bilgic, Huseyin Bilgin Bakirci, Serkan Hacilarlioglu, Selin Karagenc, Tulin Carletti, Tamara Weir, William Shiels, Brian Shkap, Varda Aktas, Munir Florin‐Christensen, Mónica Schnittger, Leonhard |
| author_sort | Mira, Anabela |
| collection | INTA Digital |
| description | Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA7778 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA77782020-08-27T16:48:30Z High genetic diversity and differentiation of the babesia ovis population in Turkey Mira, Anabela Unlu, Ahmet Hakan Bilgic, Huseyin Bilgin Bakirci, Serkan Hacilarlioglu, Selin Karagenc, Tulin Carletti, Tamara Weir, William Shiels, Brian Shkap, Varda Aktas, Munir Florin‐Christensen, Mónica Schnittger, Leonhard Babesia ovis Diversidad genética (como recurso) Tipificación de Secuencias Multilocus Babesiosis Estructura de la Población ADN Satélite Turquía Ovinos Caprinos Genetic diversity (as resource) Multilocus Sequence Typing Population Structure Satellite DNA Sheep Goats Babesia ovis is a tick‐transmitted protozoan haemoparasite causing ovine babesiosis in sheep and goats leading to considerable economic loss in Turkey and neighbouring countries. There are no vaccines available, therapeutic drugs leave toxic residues in meat and milk, and tick vector control entails environmental risks. A panel of eight mini‐ and micro‐satellite marker loci was developed and applied to study genetic diversity and substructuring of B. ovis from western, central and eastern Turkey. A high genetic diversity (He = 0.799) was found for the sample of overall B. ovis population (n = 107) analyzed. Principle component analysis (PCoA) revealed the existence of three parasite subpopulations: (a) a small subpopulation of isolates from Aydin, western Turkey; (b) a second cluster predominantly generated by isolates from western Turkey; and (c) a third cluster predominantly formed by isolates from central and eastern Turkey. Two B. ovis isolates from Israel included in the analysis clustered with isolates from central and eastern Turkey. This finding strongly suggests substructuring of a major Turkish population into western versus central–eastern subpopulations, while the additional smaller B. ovis population found in Aydin could have been introduced, more recently, to Turkey. STRUCTURE analysis suggests a limited exchange of parasite strains between the western and the central–eastern regions and vice versa, possibly due to limited trading of sheep. Importantly, evidence for recombinant genotypes was obtained in regionally interchanged parasite isolates. Important climatic differences between the western and the central/eastern region, with average yearly temperatures of 21°C versus 15°C, correspond with the identified geographical substructuring. We hypothesize that the different climatic conditions may result in variation in the activity of subpopulations of Rhipicephalus spp. tick vectors, which, in turn, could selectively maintain and transmit different parasite populations. These findings may have important implications for vaccine development and the spread of drug resistance. Instituto de Patobiología Fil: Mira, Anabela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Unlu, Ahmet Hakan. Van Yuzuncu Yil University. Vocational School of Gevas; Turquía Fil: Bilgic, Huseyin Bilgin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Bakirci, Serkan. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Hacilarlioglu, Selin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Karagenc, Tulin. Aydin Adnan Menderes University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Carletti, Tamara. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Weir, William. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Shiels, Brian. Universityof Glasgow. College of Medical, Veterinary and Life Sciences; Reino Unido Fil: Shkap, Varda. Kimron Veterinary Institute. Division of Parasitology; Israel Fil: Aktas, Munir. Firat University. Faculty of Veterinary Medicine. Department of Parasitology; Turquía Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2020-08-27T16:41:26Z 2020-08-27T16:41:26Z 2020-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/7778 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13174 1865-1674 https://doi.org/10.1111/tbed.13174 eng info:eu-repograntAgreement/INTA/PNBIO/1131034/AR./Inmunología molecular y genómica funcional aplicadas a interacciones patógeno hospedador de interés pecuario. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Transboundary and Emerging Diseases 67 (Supl. 2) : 26-35 (Julio 2020) |
| spellingShingle | Babesia ovis Diversidad genética (como recurso) Tipificación de Secuencias Multilocus Babesiosis Estructura de la Población ADN Satélite Turquía Ovinos Caprinos Genetic diversity (as resource) Multilocus Sequence Typing Population Structure Satellite DNA Sheep Goats Mira, Anabela Unlu, Ahmet Hakan Bilgic, Huseyin Bilgin Bakirci, Serkan Hacilarlioglu, Selin Karagenc, Tulin Carletti, Tamara Weir, William Shiels, Brian Shkap, Varda Aktas, Munir Florin‐Christensen, Mónica Schnittger, Leonhard High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title | High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title_full | High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title_fullStr | High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title_full_unstemmed | High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title_short | High genetic diversity and differentiation of the babesia ovis population in Turkey |
| title_sort | high genetic diversity and differentiation of the babesia ovis population in turkey |
| topic | Babesia ovis Diversidad genética (como recurso) Tipificación de Secuencias Multilocus Babesiosis Estructura de la Población ADN Satélite Turquía Ovinos Caprinos Genetic diversity (as resource) Multilocus Sequence Typing Population Structure Satellite DNA Sheep Goats |
| url | http://hdl.handle.net/20.500.12123/7778 https://onlinelibrary.wiley.com/doi/full/10.1111/tbed.13174 https://doi.org/10.1111/tbed.13174 |
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