Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
Here, we characterize two novel GH5 endoglucanases (GH5CelA and GH5CelB) from an uncultured bacterium identified in termite gut microbiomes. Both genes were codon-optimized, synthetized, cloned, and expressed as recombinant proteins in Escherichia coli for subsequent purification. Both enzymes showe...
| Autores principales: | , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2020
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/7767 https://link.springer.com/article/10.1007/s00253-020-10831-5 https://doi.org/10.1007/s00253-020-10831-5 |
Ejemplares similares: Characterization of two GH5 endoglucanases from termite microbiome using synthetic metagenomics
- A thermostable GH8 endoglucanase of Enterobacter sp. R1 is suitable for β- glucan deconstruction
- Synergic activity of Cel8Pa β-1,4 endoglucanase and Bg1Pa β-glucosidase from Paenibacillus xylanivorans A59 in beta-glucan conversio
- Characterization of a novel GH10 alkali‑thermostable xylanase from a termite microbiome
- Efecto de la fertilización nitrogenada y del genotipo sobre el rendimiento y el contenido de nitrógeno y β-glucanos en el grano de la avena (Avena sativa L.)
- Propiedades fisicoquímicas de harinas de trigo y avena de alta calidad panadera = Physicochemical properties of wheat and oat flours of high technological quality
- Diversity structure of the microbial communities in the guts of four neotropical termite species