Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina
Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this study was to determine calf, herd, and within-herd Cryptosporidium prevalence an...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2020
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/6690 https://link.springer.com/article/10.1007%2Fs00436-019-06366-y https://doi.org/10.1007/s00436-019-06366-y |
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| author | Lombardelli, Joaquín Andrés Tomazic, Mariela Luján Schnittger, Leonhard Tiranti, Karina |
| author_browse | Lombardelli, Joaquín Andrés Schnittger, Leonhard Tiranti, Karina Tomazic, Mariela Luján |
| author_facet | Lombardelli, Joaquín Andrés Tomazic, Mariela Luján Schnittger, Leonhard Tiranti, Karina |
| author_sort | Lombardelli, Joaquín Andrés |
| collection | INTA Digital |
| description | Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this study was to determine calf, herd, and within-herd Cryptosporidium prevalence and identify Cryptosporidium species and subtypes in calves with diarrhea in intensive dairy herds in central Argentina. A total of 1073 fecal samples were collected from 54 randomly selected dairy herds. Cryptosporidium-oocysts were isolated and concentrated from fecal samples using formol-ether and detected by light microscopy with the modified Ziehl-Neelsen technique. Overall prevalence of oocyst-excreting calves was found to be 25.5% (274/1073) (95% C.I. 22.9; 28.1%). Of the herds studied, 89% (48/54) included at least one infected calf, whereas within-herd prevalence ranged from the absence of infection to 57% (20/35). A highly significant association was found between the presence of diarrhea and C. parvum infection (χ2 = 55.89, p < 0.001). For species determination, genomic DNA isolated from oocyst-positive fecal samples was subjected to PCR-RFLP of the 18S rRNA gene resulting exclusively in Cryptosporidium parvum identification. C. parvum isolates of calves displaying diarrhea and high rate of excretion of oocysts were subtyped by PCR amplification and direct sequencing of the 60 kDa glycoprotein (GP60) gene. Altogether five GP60 subtypes, designated IIaA18G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA24G1R1 were identified. Interestingly, IIaA18G1R1 and IIaA20G1R1 were predominant in calves with diarrhea and high infection intensity. Notably, IIaA24G1R1 represents a novel, previously unrecognized C. parvum subtype. The subtype IIaA18G1R1, frequently found in this study, is strongly implicated in zoonotic transmission. These results suggest that calves might be an important source for human cryptosporidiosis in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6690 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA66902020-01-16T14:39:46Z Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina Lombardelli, Joaquín Andrés Tomazic, Mariela Luján Schnittger, Leonhard Tiranti, Karina Cryptosporidium Parvum Diarrea Transmisión de Enfermedades Ternero Argentina Diarrhoea Disease Transmission Calves GP60 Subtype Cryptosporidiosis of calves is caused by the enteroprotozoan Cryptosporidium spp. The disease results in intense diarrhea of calves associated with substantial economic losses in dairy farming worldwide. The aim of this study was to determine calf, herd, and within-herd Cryptosporidium prevalence and identify Cryptosporidium species and subtypes in calves with diarrhea in intensive dairy herds in central Argentina. A total of 1073 fecal samples were collected from 54 randomly selected dairy herds. Cryptosporidium-oocysts were isolated and concentrated from fecal samples using formol-ether and detected by light microscopy with the modified Ziehl-Neelsen technique. Overall prevalence of oocyst-excreting calves was found to be 25.5% (274/1073) (95% C.I. 22.9; 28.1%). Of the herds studied, 89% (48/54) included at least one infected calf, whereas within-herd prevalence ranged from the absence of infection to 57% (20/35). A highly significant association was found between the presence of diarrhea and C. parvum infection (χ2 = 55.89, p < 0.001). For species determination, genomic DNA isolated from oocyst-positive fecal samples was subjected to PCR-RFLP of the 18S rRNA gene resulting exclusively in Cryptosporidium parvum identification. C. parvum isolates of calves displaying diarrhea and high rate of excretion of oocysts were subtyped by PCR amplification and direct sequencing of the 60 kDa glycoprotein (GP60) gene. Altogether five GP60 subtypes, designated IIaA18G1R1, IIaA20G1R1, IIaA21G1R1, IIaA22G1R1, and IIaA24G1R1 were identified. Interestingly, IIaA18G1R1 and IIaA20G1R1 were predominant in calves with diarrhea and high infection intensity. Notably, IIaA24G1R1 represents a novel, previously unrecognized C. parvum subtype. The subtype IIaA18G1R1, frequently found in this study, is strongly implicated in zoonotic transmission. These results suggest that calves might be an important source for human cryptosporidiosis in Argentina. Instituto de Patobiología Fil: Lombardelli Joaquín Andrés. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina Fil: Tiranti, Karina. Universidad Nacional de Río Cuarto. Departamento de Patología Animal; Argentina 2020-01-16T14:25:54Z 2020-01-16T14:25:54Z 2019-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6690 https://link.springer.com/article/10.1007%2Fs00436-019-06366-y 1432-1955 (Online) 0932-0113 https://doi.org/10.1007/s00436-019-06366-y eng info:eu-repo/semantics/restrictedAccess application/pdf Springer Parasitology Research 118 (7) : 2079-2086. (July 2019) |
| spellingShingle | Cryptosporidium Parvum Diarrea Transmisión de Enfermedades Ternero Argentina Diarrhoea Disease Transmission Calves GP60 Subtype Lombardelli, Joaquín Andrés Tomazic, Mariela Luján Schnittger, Leonhard Tiranti, Karina Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title | Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title_full | Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title_fullStr | Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title_full_unstemmed | Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title_short | Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina |
| title_sort | prevalence of cryptosporidium parvum in dairy calves and gp60 subtyping of diarrheic calves in central argentina |
| topic | Cryptosporidium Parvum Diarrea Transmisión de Enfermedades Ternero Argentina Diarrhoea Disease Transmission Calves GP60 Subtype |
| url | http://hdl.handle.net/20.500.12123/6690 https://link.springer.com/article/10.1007%2Fs00436-019-06366-y https://doi.org/10.1007/s00436-019-06366-y |
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