L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus
Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive toC.fetus fetus and C. fetus venerealis biovar intermedius. This gas that c...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2020
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/6617 https://peerj.com/articles/7820/ https://doi.org/10.7717/peerj.7820 |
| _version_ | 1855035708389982208 |
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| author | Farace, Pablo Daniel Morsella, Claudia Graciela Cravero, Silvio Lorenzo Pedro Sioya, Bernardo Arturo Amadio, Ariel Paolicchi, Fernando Gioffre, Andrea |
| author_browse | Amadio, Ariel Cravero, Silvio Lorenzo Pedro Farace, Pablo Daniel Gioffre, Andrea Morsella, Claudia Graciela Paolicchi, Fernando Sioya, Bernardo Arturo |
| author_facet | Farace, Pablo Daniel Morsella, Claudia Graciela Cravero, Silvio Lorenzo Pedro Sioya, Bernardo Arturo Amadio, Ariel Paolicchi, Fernando Gioffre, Andrea |
| author_sort | Farace, Pablo Daniel |
| collection | INTA Digital |
| description | Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive toC.fetus fetus and C. fetus venerealis biovar intermedius. This gas that can be biochemically tested can be produced from L-cysteine (L-Cys). Herein, we report a novel multiplex-PCR to differentiate C. fetus based on the evaluation of a deletion of an ATP-binding cassette-type L-Cys transporter that could be involved in hydrogen sulfide production, as previously described. A wet lab approach combined with an in silico whole genome data analysis showed complete agreement between this L-Cys transporter-PCR and the hydrogen sulfide production biochemical test. This multiplex-PCR may complement the tests currently employed for the differential diagnosis of C. fetus. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6617 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | INTA66172020-01-06T17:55:27Z L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus Farace, Pablo Daniel Morsella, Claudia Graciela Cravero, Silvio Lorenzo Pedro Sioya, Bernardo Arturo Amadio, Ariel Paolicchi, Fernando Gioffre, Andrea PCR Campylobacteriosis Campylobacter Fetus Sulfide Production Molecular Differentiation Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive toC.fetus fetus and C. fetus venerealis biovar intermedius. This gas that can be biochemically tested can be produced from L-cysteine (L-Cys). Herein, we report a novel multiplex-PCR to differentiate C. fetus based on the evaluation of a deletion of an ATP-binding cassette-type L-Cys transporter that could be involved in hydrogen sulfide production, as previously described. A wet lab approach combined with an in silico whole genome data analysis showed complete agreement between this L-Cys transporter-PCR and the hydrogen sulfide production biochemical test. This multiplex-PCR may complement the tests currently employed for the differential diagnosis of C. fetus. Instituto de Biotecnología Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Laboratorio de Bacteriología-Grupo de Sanidad Animal. Universidad Nacional de Mar del Plata; Argentina Fil: Cravero, Silvio Lorenzo Pedro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Sioya, Bernardo Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Unidad Integrada. Laboratorio de Bacteriología-Grupo de Sanidad Animal. Universidad Nacional de Mar del Plata; Argentina Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina 2020-01-06T17:48:58Z 2020-01-06T17:48:58Z 2019-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/6617 https://peerj.com/articles/7820/ 2167-8359 https://doi.org/10.7717/peerj.7820 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PeerJ. 7 : e7820. (2019) |
| spellingShingle | PCR Campylobacteriosis Campylobacter Fetus Sulfide Production Molecular Differentiation Farace, Pablo Daniel Morsella, Claudia Graciela Cravero, Silvio Lorenzo Pedro Sioya, Bernardo Arturo Amadio, Ariel Paolicchi, Fernando Gioffre, Andrea L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title | L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title_full | L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title_fullStr | L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title_full_unstemmed | L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title_short | L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus |
| title_sort | l cysteine transporter pcr to detect hydrogen sulfide producing campylobacter fetus |
| topic | PCR Campylobacteriosis Campylobacter Fetus Sulfide Production Molecular Differentiation |
| url | http://hdl.handle.net/20.500.12123/6617 https://peerj.com/articles/7820/ https://doi.org/10.7717/peerj.7820 |
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