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L-cysteine transporter-PCR to detect hydrogen sulfide-producing Campylobacter fetus

Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive toC.fetus fetus and C. fetus venerealis biovar intermedius. This gas that c...

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Main Authors: Farace, Pablo Daniel, Morsella, Claudia Graciela, Cravero, Silvio Lorenzo Pedro, Sioya, Bernardo Arturo, Amadio, Ariel, Paolicchi, Fernando, Gioffre, Andrea
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2020
Subjects:
PCR
Campylobacteriosis
Campylobacter Fetus
Sulfide Production
Molecular Differentiation
Online Access:http://hdl.handle.net/20.500.12123/6617
https://peerj.com/articles/7820/
https://doi.org/10.7717/peerj.7820
Summary:Phenotypic differences between Campylobacter fetus fetus and C. fetus venerealis subspecies allow the differential diagnosis of bovine genital campylobacteriosis. The hydrogensulfide production,for example,is atrait exclusive toC.fetus fetus and C. fetus venerealis biovar intermedius. This gas that can be biochemically tested can be produced from L-cysteine (L-Cys). Herein, we report a novel multiplex-PCR to differentiate C. fetus based on the evaluation of a deletion of an ATP-binding cassette-type L-Cys transporter that could be involved in hydrogen sulfide production, as previously described. A wet lab approach combined with an in silico whole genome data analysis showed complete agreement between this L-Cys transporter-PCR and the hydrogen sulfide production biochemical test. This multiplex-PCR may complement the tests currently employed for the differential diagnosis of C. fetus.

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