Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes
The use of modified atmospheres has been successfully applied in different fruits to delay the ripening process and to prevent physiological disorders. In addition, during normal ripening, hypoxic areas are generated inside the fruit; moreover, anaerobic conditions may also arise during fruit post-h...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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Oxford Academic Press
2019
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| Subjects: | |
| Online Access: | https://academic.oup.com/pcp/article/52/2/392/1906254 http://hdl.handle.net/20.500.12123/6476 https://doi.org/10.1093/pcp/pcq200 |
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| author | Lara, María Valeria Budde, Claudio Olaf Porrini, Lucía Borsani, Julia Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana |
| author_browse | Andreo, Carlos Santiago Borsani, Julia Budde, Claudio Olaf Drincovich, María Fabiana Lara, María Valeria Murray, Ricardo Ernesto Porrini, Lucía |
| author_facet | Lara, María Valeria Budde, Claudio Olaf Porrini, Lucía Borsani, Julia Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana |
| author_sort | Lara, María Valeria |
| collection | INTA Digital |
| description | The use of modified atmospheres has been successfully applied in different fruits to delay the ripening process and to prevent physiological disorders. In addition, during normal ripening, hypoxic areas are generated inside the fruit; moreover, anaerobic conditions may also arise during fruit post-harvest storage and handling. In consequence, the fruit is an interesting model to analyze the metabolic modifications due to changes in oxygen levels. In this work, a 72 h anoxic treatment by using an N2 storage atmosphere was applied to peaches (Prunus persica L. Batsch) after harvest. Ripening was effectively delayed in treated fruits, preventing fruit softening, color changes and ethylene production. Metabolic changes induced by anoxia included induction of fermentative pathways, glycolysis and enzymes involved in both sucrose synthesis and degradation. Sucrose, fructose and glucose contents remained unchanged in treated fruit, probably due to sucrose cycling. Sorbitol was not consumed and citrate was increased, correlating with citric acid cycle impairment due to O2 deprivation. Malate content was not affected, indicating compensation in the reactions producing and consuming malate. Changes in malic enzymes and pyruvate orthophosphate dikinase may provide pyruvate for fermentation or even act to regenerate NADP. After fruit transfer to aerobic conditions, no signs of post-anoxia injury were observed and metabolic changes were reversed, with the exception of acetaldehyde levels. The results obtained indicate that peach fruit is an organ with a high capacity for anoxic tolerance, which is in accord with the presence of hypoxic areas inside fruits and the fact that hypoxic pre-treatment improves tolerance to subsequent anoxia. |
| format | info:ar-repo/semantics/artículo |
| id | INTA6476 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Oxford Academic Press |
| publisherStr | Oxford Academic Press |
| record_format | dspace |
| spelling | INTA64762019-12-09T15:36:30Z Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes Lara, María Valeria Budde, Claudio Olaf Porrini, Lucía Borsani, Julia Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Durazno Prunus Persica Anoxia Maduramiento Enzimas Peaches Ripening Enzymes Deficiencia de Oxígeno The use of modified atmospheres has been successfully applied in different fruits to delay the ripening process and to prevent physiological disorders. In addition, during normal ripening, hypoxic areas are generated inside the fruit; moreover, anaerobic conditions may also arise during fruit post-harvest storage and handling. In consequence, the fruit is an interesting model to analyze the metabolic modifications due to changes in oxygen levels. In this work, a 72 h anoxic treatment by using an N2 storage atmosphere was applied to peaches (Prunus persica L. Batsch) after harvest. Ripening was effectively delayed in treated fruits, preventing fruit softening, color changes and ethylene production. Metabolic changes induced by anoxia included induction of fermentative pathways, glycolysis and enzymes involved in both sucrose synthesis and degradation. Sucrose, fructose and glucose contents remained unchanged in treated fruit, probably due to sucrose cycling. Sorbitol was not consumed and citrate was increased, correlating with citric acid cycle impairment due to O2 deprivation. Malate content was not affected, indicating compensation in the reactions producing and consuming malate. Changes in malic enzymes and pyruvate orthophosphate dikinase may provide pyruvate for fermentation or even act to regenerate NADP. After fruit transfer to aerobic conditions, no signs of post-anoxia injury were observed and metabolic changes were reversed, with the exception of acetaldehyde levels. The results obtained indicate that peach fruit is an organ with a high capacity for anoxic tolerance, which is in accord with the presence of hypoxic areas inside fruits and the fact that hypoxic pre-treatment improves tolerance to subsequent anoxia. EEA San Pedro Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Porrini, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Bosani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Murray, Ricardo Ernesto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Tecnológico de Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina 2019-12-09T15:35:07Z 2019-12-09T15:35:07Z 2011-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/pcp/article/52/2/392/1906254 http://hdl.handle.net/20.500.12123/6476 0032-0781 1471-9053 https://doi.org/10.1093/pcp/pcq200 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Oxford Academic Press Plant and Cell Physiology 52 (2) : 392–403 (February 2011) |
| spellingShingle | Durazno Prunus Persica Anoxia Maduramiento Enzimas Peaches Ripening Enzymes Deficiencia de Oxígeno Lara, María Valeria Budde, Claudio Olaf Porrini, Lucía Borsani, Julia Murray, Ricardo Ernesto Andreo, Carlos Santiago Drincovich, María Fabiana Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title | Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title_full | Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title_fullStr | Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title_full_unstemmed | Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title_short | Peach (Prunus Persica) Fruit Response to Anoxia: Reversible Ripening Delay and Biochemical Changes |
| title_sort | peach prunus persica fruit response to anoxia reversible ripening delay and biochemical changes |
| topic | Durazno Prunus Persica Anoxia Maduramiento Enzimas Peaches Ripening Enzymes Deficiencia de Oxígeno |
| url | https://academic.oup.com/pcp/article/52/2/392/1906254 http://hdl.handle.net/20.500.12123/6476 https://doi.org/10.1093/pcp/pcq200 |
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