Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative aty...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/629 https://www.karger.com/Article/Abstract/451056 https://doi.org/10.1159/000451056 |
| _version_ | 1855482756972150784 |
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| author | Benintende, Graciela Beatriz Berretta, Marcelo Facundo Zandomeni, Ruben Sauka, Diego Hernan Amadio, Ariel F. Ortiz, Elio Maximiliano Navas, Laura Emilce |
| author_browse | Amadio, Ariel F. Benintende, Graciela Beatriz Berretta, Marcelo Facundo Navas, Laura Emilce Ortiz, Elio Maximiliano Sauka, Diego Hernan Zandomeni, Ruben |
| author_facet | Benintende, Graciela Beatriz Berretta, Marcelo Facundo Zandomeni, Ruben Sauka, Diego Hernan Amadio, Ariel F. Ortiz, Elio Maximiliano Navas, Laura Emilce |
| author_sort | Benintende, Graciela Beatriz |
| collection | INTA Digital |
| description | We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2
vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity
island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624. |
| format | Artículo |
| id | INTA629 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA6292019-03-22T12:10:59Z Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes Benintende, Graciela Beatriz Berretta, Marcelo Facundo Zandomeni, Ruben Sauka, Diego Hernan Amadio, Ariel F. Ortiz, Elio Maximiliano Navas, Laura Emilce Genética Bacillus Thuringiensis Genes Genetics pFR260 Genes Insecticidas We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624. EEA Rafaela Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zandomeni, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ortiz, Elio Maximiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Navas, Laura Emilce. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-07-10T18:07:44Z 2017-07-10T18:07:44Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/629 https://www.karger.com/Article/Abstract/451056 1660-2412 https://doi.org/10.1159/000451056 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of molecular microbiology and biotechnology 27 : 43-54. (2017) |
| spellingShingle | Genética Bacillus Thuringiensis Genes Genetics pFR260 Genes Insecticidas Benintende, Graciela Beatriz Berretta, Marcelo Facundo Zandomeni, Ruben Sauka, Diego Hernan Amadio, Ariel F. Ortiz, Elio Maximiliano Navas, Laura Emilce Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title | Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title_full | Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title_fullStr | Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title_full_unstemmed | Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title_short | Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes |
| title_sort | complete sequence and organization of pfr260 the bacillus thuringiensis inta fr7 4 plasmid harboring insecticidal genes |
| topic | Genética Bacillus Thuringiensis Genes Genetics pFR260 Genes Insecticidas |
| url | http://hdl.handle.net/20.500.12123/629 https://www.karger.com/Article/Abstract/451056 https://doi.org/10.1159/000451056 |
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