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Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes

We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative aty...

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Autores principales: Benintende, Graciela Beatriz, Berretta, Marcelo Facundo, Zandomeni, Ruben, Sauka, Diego Hernan, Amadio, Ariel F., Ortiz, Elio Maximiliano, Navas, Laura Emilce
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Genética
Bacillus Thuringiensis
Genes
Genetics
pFR260
Genes Insecticidas
Acceso en línea:http://hdl.handle.net/20.500.12123/629
https://www.karger.com/Article/Abstract/451056
https://doi.org/10.1159/000451056
Sumario:We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.

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