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Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes

We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative aty...

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Main Authors: Benintende, Graciela Beatriz, Berretta, Marcelo Facundo, Zandomeni, Ruben, Sauka, Diego Hernan, Amadio, Ariel F., Ortiz, Elio Maximiliano, Navas, Laura Emilce
Format: Artículo
Language:Inglés
Published: 2017
Subjects:
Genética
Bacillus Thuringiensis
Genes
Genetics
pFR260
Genes Insecticidas
Online Access:http://hdl.handle.net/20.500.12123/629
https://www.karger.com/Article/Abstract/451056
https://doi.org/10.1159/000451056
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author Benintende, Graciela Beatriz
Berretta, Marcelo Facundo
Zandomeni, Ruben
Sauka, Diego Hernan
Amadio, Ariel F.
Ortiz, Elio Maximiliano
Navas, Laura Emilce
author_browse Amadio, Ariel F.
Benintende, Graciela Beatriz
Berretta, Marcelo Facundo
Navas, Laura Emilce
Ortiz, Elio Maximiliano
Sauka, Diego Hernan
Zandomeni, Ruben
author_facet Benintende, Graciela Beatriz
Berretta, Marcelo Facundo
Zandomeni, Ruben
Sauka, Diego Hernan
Amadio, Ariel F.
Ortiz, Elio Maximiliano
Navas, Laura Emilce
author_sort Benintende, Graciela Beatriz
collection INTA Digital
description We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624.
format Artículo
id INTA629
institution Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina)
language Inglés
publishDate 2017
publishDateRange 2017
publishDateSort 2017
record_format dspace
spelling INTA6292019-03-22T12:10:59Z Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes Benintende, Graciela Beatriz Berretta, Marcelo Facundo Zandomeni, Ruben Sauka, Diego Hernan Amadio, Ariel F. Ortiz, Elio Maximiliano Navas, Laura Emilce Genética Bacillus Thuringiensis Genes Genetics pFR260 Genes Insecticidas We report the complete sequence and analysis of pFR260, a novel megaplasmid of 260,595 bp from the Bacillus thuringiensis strain INTA Fr7-4 isolated in Argentina. It carries 7 insecticidal genes: 3 cry8 copies previously reported, 2 vip1, and 2 vip2 . Also, it carries a gene encoding a putative atypical Cry protein. These genes are arranged in a region of approximately 105 kbp in size with characteristics of a pathogenicity island with a potential coleopteran-specific insecticide profile. DNA strand composition asymmetry, as determined by GC skew analysis, and the presence of a Rep protein involved in the initiation of replication suggest a bidirectional theta mechanism of replication. In addition, many genes involved in conjugation and a CRISPR-Cas system were detected. The pFR260 sequence was deposited in GenBank under accession number KX258624. EEA Rafaela Fil: Benintende, Graciela Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Berretta, Marcelo Facundo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zandomeni, Rubén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sauka, Diego Hernan. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ortiz, Elio Maximiliano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Navas, Laura Emilce. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-07-10T18:07:44Z 2017-07-10T18:07:44Z 2017 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/629 https://www.karger.com/Article/Abstract/451056 1660-2412 https://doi.org/10.1159/000451056 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of molecular microbiology and biotechnology 27 : 43-54. (2017)
spellingShingle Genética
Bacillus Thuringiensis
Genes
Genetics
pFR260
Genes Insecticidas
Benintende, Graciela Beatriz
Berretta, Marcelo Facundo
Zandomeni, Ruben
Sauka, Diego Hernan
Amadio, Ariel F.
Ortiz, Elio Maximiliano
Navas, Laura Emilce
Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title_full Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title_fullStr Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title_full_unstemmed Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title_short Complete Sequence and Organization of pFR260, the Bacillus thuringiensis INTA Fr7-4 Plasmid Harboring Insecticidal Genes
title_sort complete sequence and organization of pfr260 the bacillus thuringiensis inta fr7 4 plasmid harboring insecticidal genes
topic Genética
Bacillus Thuringiensis
Genes
Genetics
pFR260
Genes Insecticidas
url http://hdl.handle.net/20.500.12123/629
https://www.karger.com/Article/Abstract/451056
https://doi.org/10.1159/000451056
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